Laboratório de Biologia Molecular de Cestódeos e Laboratório de Genômica Estrutural e Funcional, Centro de Biotecnologia, UFRGS, Porto Alegre, RS, Brazil.
Proteomics. 2010 May;10(10):1985-99. doi: 10.1002/pmic.200900506.
Cystic hydatid disease (CHD) is caused by infection with the Echinococcus granulosus metacestode and affects both humans and livestock. In this work, we performed a proteomic analysis of the E. granulosus metacestode during infection of its intermediate bovine host. Parasite proteins were identified in different metacestode components (94 from protoscolex, 25 from germinal layer and 20 from hydatid cyst fluid), along with host proteins (58) that permeate into the hydatid cyst, providing new insights into host-parasite interplay. E. granulosus and platyhelminth EST data allowed successful identification of proteins potentially involved in downregulation of host defenses, highlighting possible evasion mechanisms adopted by the parasite to establish infection. Several intracellular proteins were found in hydatid cyst fluid, revealing a set of newly identified proteins that were previously thought to be inaccessible for inducing or modulating the host immune response. Host proteins identified in association with the hydatid cyst suggest that the parasite may bind/adsorb host molecules with nutritional and/or immune evasion purposes, masking surface antigens or inhibiting important effector molecules of host immunity, such as complement components and calgranulin. Overall, our results provide valuable information on parasite survival strategies in the adverse host environment and on the molecular mechanisms underpinning CHD immunopathology.
包虫病（CHD）是由细粒棘球绦虫的幼虫感染引起的，影响人类和家畜。在这项工作中，我们对中间宿主牛感染细粒棘球蚴的过程中的棘球蚴进行了蛋白质组学分析。在不同的棘球蚴成分中鉴定出寄生虫蛋白（原头蚴 94 个，生发层 25 个，包囊液 20 个），以及渗透到包囊中的宿主蛋白（58 个），为宿主-寄生虫相互作用提供了新的见解。细粒棘球蚴和扁形动物 EST 数据成功鉴定了可能参与下调宿主防御的蛋白质，突出了寄生虫可能采用的逃避机制来建立感染。在包囊液中发现了几种细胞内蛋白，揭示了一组新鉴定的蛋白，这些蛋白以前被认为是无法诱导或调节宿主免疫反应的。与包囊相关的宿主蛋白表明，寄生虫可能具有结合/吸附宿主分子的功能，具有营养和/或免疫逃避的目的，掩盖表面抗原或抑制宿主免疫的重要效应分子，如补体成分和钙粒蛋白。总的来说，我们的研究结果提供了有关寄生虫在不利的宿主环境中生存策略以及 CHD 免疫病理学的分子机制的有价值的信息。
