Mu Lu-xia, Zhu Lu, Zhao Ai-hua, Zhou Ming-mei, Jia Wei
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
Zhong Yao Cai. 2009 Nov;32(11):1741-5.
To optimize the extraction and purification procedure for polysaccharides from Astragalus membranaceus.
The orthogonal test was used to determine the optimal extraction technology in terms of the extraction rate of crude polysaccharides and the concentration of polysaccharides. The efficiency of deproteinization and decoloration with different methods were evaluated with the ration of protein and colorant removing as the indices. Their influence on the content of Astragalus polysaccharides was calculated as well.
The optimum extraction procedure was as follows: 8 times of water extracting three times at 100 degrees C, 90 minutes per time. Deproteinization using pepsase combined with Sevage solvent and depigmenting with DEAE fibrin achieved a high purification rate.
The optimized extraction and purification procedure is simple, quick and efficient. The result establishes the foundation for the further study of the polysaccharides from Astragalus membranaceus.
优化黄芪多糖的提取与纯化工艺。
采用正交试验，以粗多糖提取率和多糖浓度为指标确定最佳提取工艺。以蛋白质去除率和色素去除率为指标，评价不同方法的脱蛋白和脱色效果，并计算其对黄芪多糖含量的影响。
最佳提取工艺为：100℃水提3次，每次90分钟，料液比1∶8。采用胃蛋白酶结合Sevage法脱蛋白、DEAE纤维素法脱色，纯化率较高。
优化后的提取与纯化工艺简便、快速、高效，为黄芪多糖的进一步研究奠定了基础。
