黄芪根中铜含量与有机成分的相关性研究

[Correlation studies of contents of copper and organic components in Astragalus roots].

作者信息

Li Shu-lan, Gu Xuan, Ma Dan, Hu Yu-zhu

机构信息

China Pharmaceutical University, Jiangsu Narjing, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2006 Aug;31(15):1249-53.

PMID:17048569

Abstract

OBJECTIVE

To study the relationship between copper and organic components, total flavonoids of Astragalus (TFA), total saponins of Astragalus (TSA) and total polysaccharide of Astragalus (TPA).

METHOD

TFA, TSA and TPA were extracted from Astragalus roots using different organic solvents, and determined by colorimetry. The concentration of copper in extracts was determined by graphite furnace atomic absorption spectrometry (GFAAS).

RESULT

Copper was found in TFA, TPA and TSA, and its concentration in TFA and TPA was higher; the content of copper was correlated significantly to that of TFA and TPA (r1(2) = 0.754 8, F1 = 57.202, P < 0.01 and r2(2) = 0.499, F2 = 21.906, P < 0.01), while not to that of TSA ( r3(2) = 0.0026, F3 = 0.041, P > 0.1).

CONCLUSION

Correlation analysis could reveal the relationship of copper with organic components in Astragalus roots; contents and structure characteristics of organic components might be important factors influencing the distribution of copper in Astragalus roots.

摘要

目的

研究铜与黄芪有机成分、黄芪总黄酮（TFA）、黄芪总皂苷（TSA）及黄芪总多糖（TPA）之间的关系。

方法

采用不同有机溶剂从黄芪根中提取TFA、TSA和TPA，并用比色法测定。提取物中铜的浓度采用石墨炉原子吸收光谱法（GFAAS）测定。

结果

在TFA、TPA和TSA中均检测到铜，且其在TFA和TPA中的浓度较高；铜含量与TFA和TPA的含量显著相关（r1(2) = 0.754 8，F1 = 57.202，P < 0.01；r2(2) = 0.499，F2 = 21.906，P < 0.01），而与TSA的含量无相关性（r3(2) = 0.0026，F3 = 0.041，P > 0.1）。