School of Biochemistry, Devi Ahilya University, Indore, India.
J Agric Food Chem. 2010 Apr 14;58(7):4298-302. doi: 10.1021/jf904228e.
Three Indian soybean genotypes, namely, Kalitur, Hara soya and NRC37 with black, green and yellow colored seed coat respectively were gamma irradiated at a dose of 0.5, 2.0, and 5.0 kGy. The total isoflavones and total phenol content (TPC) in all the genotypes increased significantly at a dose of 0.5 and 2 kGy respectively. The anthocyanin content was high in Kalitur, while other genotypes showed no detectable amounts of it. The hydroxyl radical scavenging activity (HRSA), DPPH free radical scavenging activity (FRSA) and total antioxidant power (TAP) were highest in Kalitur with black seed coat color. However, maximum enhancement in antioxidant properties was found in NRC37 with yellow followed by Hara soya with green seed coat color at a dose of 0.5 and 2.0 kGy. It was also observed that the 3 soybean genotypes showed an increase in antioxidant constituents and antioxidative properties at lower doses of 0.5 and 2.0 kGy while, the antioxidant effects of soy seeds were either decreased or remained constant at a higher dose of 5.0 kGy. It is suggested that mild gamma irradiation enhanced the antioxidant constituents and, hence, antioxidant potential of soybean seeds.
三种印度大豆基因型，即分别具有黑色、绿色和黄色种皮的 Kalitur、Hara soya 和 NRC37，在 0.5、2.0 和 5.0 kGy 的剂量下进行了伽马辐射。在 0.5 和 2 kGy 的剂量下，所有基因型的总异黄酮和总酚含量（TPC）均显著增加。Kalitur 中的花色苷含量较高，而其他基因型则没有检测到。具有黑色种皮的 Kalitur 具有最高的羟基自由基清除活性（HRSA）、DPPH 自由基清除活性（FRSA）和总抗氧化能力（TAP）。然而，在 0.5 和 2.0 kGy 的剂量下，黄色的 NRC37 和绿色的 Hara soya 的抗氧化特性增强最大。还观察到，在较低剂量的 0.5 和 2.0 kGy 下，这 3 种大豆基因型的抗氧化成分和抗氧化特性增加，而在较高剂量的 5.0 kGy 下，大豆种子的抗氧化效果要么降低要么保持不变。建议轻度伽马辐射增强了大豆种子的抗氧化成分，从而增强了其抗氧化潜力。
