Max-Planck-Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
Nano Lett. 2010 Apr 14;10(4):1497-500. doi: 10.1021/nl100593x.
We present a novel fluorescence lifetime imaging microscopy technique to measure absolute positions of fluorescent molecules within 100 nm above a metalized surface based on distance-dependent fluorescence lifetime modulations. We apply this technique to fluorescently labeled microtubules as optical probes with various unlabeled proteins attached. By measuring the fluorescence lifetimes, we obtain the position of the microtubules and therefore determine the geometrical size of the attached proteins with nanometer precision.
我们提出了一种新的荧光寿命成像显微镜技术，基于距离相关的荧光寿命调制，可在金属化表面上方 100nm 内测量荧光分子的绝对位置。我们将该技术应用于带有各种未标记蛋白的荧光标记微管作为光学探针。通过测量荧光寿命，我们获得微管的位置，从而以纳米精度确定附着蛋白的几何尺寸。
