Gorelik J, Shevchuk A, Ramalho M, Elliott M, Lei C, Higgins C F, Lab Max J, Klenerman D, Krauzewicz N, Korchev Y
Division of Medicine and Medical Research Council Clinical Sciences Centre, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London W12 0NN, United Kingdom.
Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):16018-23. doi: 10.1073/pnas.252458399. Epub 2002 Dec 4.
We have developed a method for simultaneous recording of high-resolution topography and cell surface fluorescence in a single scan which we call scanning surface confocal microscopy. The resolution of the system allows imaging of individual fluorescent particles in the nanometer range on fixed or live cells. We used this technique to record the interaction of single virus-like particles with the cell surface and demonstrated that single particles sink into the membrane in invaginations reminiscent of caveolae or pinocytic vesicles. This method provides a technique for elucidating the interaction of individual viruses and other nanoparticles, such as gene therapy vectors, with target cells. Furthermore, this technique should find widespread application for studying the relationship of fluorescently tagged molecules with components of the cell plasma membrane.
我们开发了一种在单次扫描中同时记录高分辨率形貌和细胞表面荧光的方法，我们将其称为扫描表面共聚焦显微镜。该系统的分辨率允许对固定或活细胞上纳米范围内的单个荧光颗粒进行成像。我们使用这项技术记录了单个病毒样颗粒与细胞表面的相互作用，并证明单个颗粒会沉入膜内陷中，让人联想到小窝或胞饮小泡。这种方法为阐明单个病毒和其他纳米颗粒（如基因治疗载体）与靶细胞之间的相互作用提供了一种技术。此外，这项技术在研究荧光标记分子与细胞质膜成分之间的关系方面应会有广泛应用。
