Institute of Virology, University of Zurich, Winterthurerstrasse 266a, 8057 Zurich, Switzerland.
J Virol. 2010 Jun;84(11):5583-93. doi: 10.1128/JVI.00228-10. Epub 2010 Mar 10.
Bovine herpesvirus 1 (BoHV-1) and BoHV-5 are closely related pathogens of cattle, but only BoHV-5 is considered a neuropathogen. We engineered intertypic gD exchange mutants with BoHV-1 and BoHV-5 backbones in order to address their in vitro and in vivo host ranges, with particular interest in invasion of the brain. The new viruses replicated in cell culture with similar dynamics and to titers comparable to those of their wild-type parents. However, gD of BoHV-5 (gD5) was able to interact with a surprisingly broad range of nectins. In vivo, gD5 provided a virulent phenotype to BoHV-1 in AR129 mice, featuring a high incidence of neurological symptoms and early onset of disease. However, only virus with the BoHV-5 backbone, independent of the gD type, was detected in the brain by immunohistology. Thus, gD of BoHV-5 confers an extended cellular host range to BoHV-1 and may be considered a virulence factor but does not contribute to the invasion of the brain.
牛疱疹病毒 1（BoHV-1）和 BoHV-5 是牛的密切相关病原体，但只有 BoHV-5 被认为是神经病原体。我们构建了具有 BoHV-1 和 BoHV-5 骨架的同种型 gD 交换突变体，以研究它们在体外和体内的宿主范围，特别关注对大脑的入侵。新病毒在细胞培养中以相似的动力学复制，达到与野生型亲本相当的滴度。然而，BoHV-5 的 gD（gD5）能够与出乎意料广泛的 nectin 相互作用。在体内，gD5 使 AR129 小鼠中的 BoHV-1 表现出毒力表型，具有高发生率的神经症状和疾病的早期发作。然而，只有具有 BoHV-5 骨架的病毒，而不管 gD 类型如何，通过免疫组织化学在大脑中被检测到。因此，BoHV-5 的 gD 赋予 BoHV-1 扩展的细胞宿主范围，并且可以被认为是一种毒力因子，但不有助于大脑的入侵。
