实时逆转录聚合酶链反应快速检测 2009 年甲型流感（H1N1）病毒神经氨酸酶耐药突变 H275Y。
Rapid detection of influenza A pandemic (H1N1) 2009 virus neuraminidase resistance mutation H275Y by real-time reverse transcriptase PCR.
机构信息
Central Virology Laboratory, Public Health Services, Ministry of Health, Chaim Sheba Medical Center, Tel-Hashomer 52621, Israel.
出版信息
J Clin Microbiol. 2010 May;48(5):1884-7. doi: 10.1128/JCM.02540-09. Epub 2010 Mar 10.
Abstract
The emergence of oseltamivir-resistant influenza A pandemic (H1N1) 2009 virus highlights the need for rapid oseltamivir resistance screening. We report the development and validation of high-throughput real-time reverse transcriptase PCR assays for the detection of the H275Y substitution in the neuraminidase 1 gene that can be accomplished in 3 to 4 h.
摘要
奥司他韦耐药的甲型 H1N1 流感大流行病毒的出现凸显了快速奥司他韦耐药性筛选的必要性。我们报告了用于检测神经氨酸酶 1 基因中的 H275Y 取代的高通量实时逆转录 PCR 检测方法的开发和验证，该方法可以在 3 到 4 小时内完成。
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