Central Virology Laboratory, Public Health Services, Ministry of Health, Chaim Sheba Medical Center, Tel-Hashomer 52621, Israel.
J Clin Microbiol. 2010 May;48(5):1884-7. doi: 10.1128/JCM.02540-09. Epub 2010 Mar 10.
The emergence of oseltamivir-resistant influenza A pandemic (H1N1) 2009 virus highlights the need for rapid oseltamivir resistance screening. We report the development and validation of high-throughput real-time reverse transcriptase PCR assays for the detection of the H275Y substitution in the neuraminidase 1 gene that can be accomplished in 3 to 4 h.
奥司他韦耐药的甲型 H1N1 流感大流行病毒的出现凸显了快速奥司他韦耐药性筛选的必要性。我们报告了用于检测神经氨酸酶 1 基因中的 H275Y 取代的高通量实时逆转录 PCR 检测方法的开发和验证,该方法可以在 3 到 4 小时内完成。