Peng Shuhong, Huang Liping, Gao Xiaoheng, Zhang Su, Yu Riyue, Liu Hongning, Xu Ningying
Animal Science College of Zhejiang University, Hangzhou 310029, China.
Zhongguo Zhong Yao Za Zhi. 2009 Dec;34(23):3064-7.
To investigate the effect of six cold traditional Chinese medicine on the energy metabolism factors in rats skeletal muscle.
The activity of Na(+)-K(+) -ATPase, Ca(2+) -ATPase and succinate dehydrogenase (SDH), the content of muscle glycogen, and the mRNA expression of skeletal muscle uncoupling protein 3 (UCP3) were measured after rats having been administrated with water extracts of Radix Sophorae Flavescentis, Fructus Gardeniae, Cortex Phellodendri, Radix Scutellariae, Rhizoma Coptidis, and Radix Gentianae respectively at the dose of 6.0, 7.0, 8.4, 6.0, 7.0, 4.0 g x kg(-1) for 30 days.
The activity of Na(+) -K(+) -ATPase has been depressed significantly and the content of skeletal muscle glycogen has been increased remarkably by six cold traditional Chinese medicine. The decreased tendency has been found on activity of Ca(2+) -ATPase and SDH, only the Radix scutellariaeg group decreased the Ca(2+) -ATPase activity significantly (P < 0.05), the SDH activity was decreased high significantly by Radix scutellariae, Cortex Phellodendri, Radix Gentianae and significantly by Rhizoma Coptidis. The mRNA expression of UCP3 has been decreased high significantly by all five cold traditional Chinese medicine except Cortex Phellodendri group with the decreased tendency of UCP3 mRNA expression.
The cold traditional Chinese medicine has the significant effects on the skeletal muscle energy metabolism by decreasing the utlization of the glucose and the activity of mitochondria SDH to reduce the production of ATP, and depressing the activity of Na(+)-K(+) -ATPase and Ca(2+) -ATPase to cut down the consumption of ATP, by decreasing the mRNA expression of UCP3 to decrease the heat production.
探讨六味寒性中药对大鼠骨骼肌能量代谢相关因子的影响。
分别以6.0、7.0、8.4、6.0、7.0、4.0 g·kg⁻¹的剂量给予大鼠苦参、栀子、黄柏、黄芩、黄连、龙胆的水提取物，连续给药30天后，检测大鼠骨骼肌中钠钾ATP酶（Na⁺-K⁺-ATPase）、钙ATP酶（Ca²⁺-ATPase）、琥珀酸脱氢酶（SDH）的活性、肌糖原含量以及骨骼肌解偶联蛋白3（UCP3）的mRNA表达。
六味寒性中药均能显著降低Na⁺-K⁺-ATPase的活性，显著增加骨骼肌糖原含量。Ca²⁺-ATPase和SDH活性呈降低趋势，仅黄芩组Ca²⁺-ATPase活性显著降低（P<0.05），黄芩、黄柏、龙胆组SDH活性显著降低，黄连组SDH活性极显著降低。除黄柏组UCP3 mRNA表达呈降低趋势外，其余五味寒性中药均极显著降低UCP3 mRNA表达。
寒性中药可通过降低葡萄糖利用率及线粒体SDH活性以减少ATP生成，抑制Na⁺-K⁺-ATPase和Ca²⁺-ATPase活性以减少ATP消耗，降低UCP3 mRNA表达以减少产热，从而对骨骼肌能量代谢产生显著影响。
