Immunalysis Corporation, 829 Towne Center Drive, Pomona, California 91767, USA.
J Anal Toxicol. 2010 Mar;34(2):64-72. doi: 10.1093/jat/34.2.64.
An analytical procedure for the determination of widely prescribed drugs for the treatment of depression and anxiety disorders, including amitriptyline, cyclobenzaprine, imipramine, dothiepin, doxepin, fluoxetine, sertraline, trimipramine, protriptyline, chlorpromazine, clomipramine, and some of their metabolites (nortriptyline, desmethyldoxepin, desipramine, desmethyltrimipramine, norclomipramine) in oral fluid has been developed and validated using liquid chromatography with tandem mass spectral detection. The oral fluid samples were collected using the Quantisal device and screened with enzyme-linked immunosorbent assay. Any drugs present were quantified using mixed-mode solid-phase extraction followed by mass spectrometric detection in positive electrospray ionization mode. For confirmation, two transitions were monitored, and the ratio between the two was required to be within 20% of the known calibration standard. Because of the worldwide shortage of acetonitrile, which was first reported in October 2008, the mobile phase was optimized to use methanol as the organic component. For all compounds, the lower limit of quantitation was 5 ng/mL; the intraday precision ranged from 2.9 to 8.2% (n = 6); interday precision from 1.5 to 6.2% (n = 30) at a concentration of 40 ng/mL. The percentage recovery of antidepressants from the oral fluid collection pad was calculated at a concentration of 40 ng/mL and ranged from 51.4 to 84.1% (n = 6). The aim of the study was to develop a confirmatory procedure for drugs in oral fluid that had been identified as presumptively positive for antidepressants and related compounds. The methods were applied to research oral fluid specimens received into our facility for testing.
已经开发并验证了一种分析程序，用于测定广泛用于治疗抑郁和焦虑障碍的处方药物，包括阿米替林、环苯扎林、丙咪嗪、多噻平、多虑平、氟西汀、舍曲林、曲米帕明、普罗替林、氯丙嗪、氯米帕明和它们的一些代谢物（去甲替林、去甲多塞平、去甲丙咪嗪、去甲曲米帕明、去甲氯米帕明）在口服液中的含量，该方法使用液相色谱-串联质谱检测法。采用 Quantisal 装置采集口服液样本，并使用酶联免疫吸附测定法进行筛选。使用混合模式固相萃取法对存在的任何药物进行定量，然后在正电喷雾电离模式下进行质谱检测。用于确证时，监测两个转换，两个转换之间的比值需要在已知校准标准的 20%以内。由于 2008 年 10 月首次报道的乙腈全球短缺，对流动相进行了优化，使用甲醇作为有机成分。对于所有化合物，定量下限均为 5 ng/mL；在 40ng/mL 浓度下，日内精密度范围为 2.9%至 8.2%（n=6）；日间精密度为 1.5%至 6.2%（n=30）。在 40ng/mL 浓度下，从口服液采集垫中回收抗抑郁药的百分比为 51.4%至 84.1%（n=6）。该研究的目的是开发一种用于确认口服液中已被确认为抗抑郁药和相关化合物阳性的药物的确认程序。该方法应用于我们机构收到用于检测的研究口服液标本。
