Instituto de Fermentaciones Industriales (CSIC), Juan de la Cierva, 3. 28006. Madrid, Spain.
Int J Food Microbiol. 2010 May 30;140(1):14-8. doi: 10.1016/j.ijfoodmicro.2010.02.021. Epub 2010 Feb 23.
The effect of growth phase on the adherence to and invasion of Caco-2 epithelial cells by five strains of Campylobacter was studied. No significant differences were observed between the behaviors in the exponential or stationary phases for the most stationary-phase tolerant strains (C. jejuni 118 and C. coli LP2), while the strains that produced a greater reduction in the viability in the stationary phase (C. jejuni 11351, C. jejuni 11168 and C. jejuni LP1), also presented reduced adherence to and invasion of Caco-2 cells. In order to find a possible explanation for the observed differences, the presence of putative virulence factors was studied in the analyzed strains. In spite of the fact that C. jejuni 118 and C. jejuni 11168 strains showed a different adherence to and invasion of Caco-2 cells behavior, they posses identical alleles for ciaB, cadF, and pldA loci. From the virulence factors analyzed, only the flaA locus was different among both strains.
研究了生长阶段对 5 株弯曲菌属黏附并侵袭 Caco-2 上皮细胞的影响。对于最耐静止期的菌株（空肠弯曲菌 118 和大肠弯曲菌 LP2），在指数期或静止期的行为之间没有观察到显著差异，而在静止期生存力降低较大的菌株（空肠弯曲菌 11351、空肠弯曲菌 11168 和空肠弯曲菌 LP1），对 Caco-2 细胞的黏附和侵袭也减少。为了找到观察到的差异的可能解释，研究了分析菌株中存在的假定毒力因子。尽管空肠弯曲菌 118 和空肠弯曲菌 11168 菌株对 Caco-2 细胞的黏附和侵袭行为不同，但它们在 ciaB、cadF 和 pldA 基因座上具有相同的等位基因。在所分析的毒力因子中，只有 flaA 基因座在这两个菌株之间存在差异。
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