Instituto de Fermentaciones Industriales (CSIC), Juan de la Cierva, 3. 28006 Madrid, Spain.
Int J Food Microbiol. 2010 Feb 28;137(2-3):181-5. doi: 10.1016/j.ijfoodmicro.2009.12.007. Epub 2009 Dec 13.
The ability of pectic oligosaccharides (POS) to inhibit adherence to and invasion of undifferentiated (UC) and differentiated (DC) Caco-2 cells by Campylobacter jejuni (C. jejuni) was investigated. It was observed that both adherence and invasion were significantly higher in UC than in DC. POS (2.5mg/ml) had no significant effect on the number of bacteria which can adhere to cells, but they significantly inhibited cell invasion. The extent of the anti-invasive effect of POS was dependent on the concentration, although the entire range tested (from 2.5mg/ml to 0.05 mg/ml) was capable of inhibiting the invasion of Caco-2 cells by Campylobacter to some degree. The pre-incubation or not of C jejuni with POS did not influence the behaviour observed. The results obtained in this work suggest that POS could be potentially useful as alternatives to antibiotics in the control of C. jejuni.
研究了果胶寡糖（POS）抑制弯曲杆菌（C. jejuni）黏附和侵袭未分化（UC）和分化（DC）Caco-2 细胞的能力。结果发现，UC 细胞的黏附和侵袭率明显高于 DC 细胞。POS（2.5mg/ml）对黏附于细胞的细菌数量没有显著影响，但显著抑制了细胞侵袭。POS 的抗侵袭作用程度取决于其浓度，尽管所测试的整个范围（从 2.5mg/ml 到 0.05mg/ml）都能够在一定程度上抑制弯曲杆菌对 Caco-2 细胞的侵袭。POS 预处理或不预处理弯曲杆菌并不影响观察到的行为。本研究结果表明，POS 可作为抗生素的替代品，用于控制 C. jejuni。
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