副产物提取物的抗氧化活性及其对[具体菌种名称1]和[具体菌种名称2]黏附、侵袭的影响以及对[相关基因名称1]和[相关基因名称2]相对表达的影响。 （注：原文中部分内容缺失具体信息，以上译文为补充完整关键信息后的表述）
Antioxidant activity and influence of byproduct extracts on adherence and invasion of and on the relative expression of and .
作者信息
Castillo Sandra, Dávila-Aviña Jorge, Heredia Norma, Garcia Santos
机构信息
Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Apdo. Postal 124-F, San Nicolás, Nuevo León 66451 México.
出版信息
Food Sci Biotechnol. 2017 Apr 30;26(2):453-459. doi: 10.1007/s10068-017-0062-x. eCollection 2017.
Adherence and invasion to cells are the key processes during infection development by (). In this study, extracts from the byproducts of , , and were added to the cultures of , and the adherence and invasion of to HeLa cells and the expression of and genes were analyzed. The relative expression of the genes was determined by quantitative reverse transcription PCR (qRT-PCR). The antioxidant activity was determined using spectrophotometric methods. Byproduct extracts at subinhibitory concentrations affected the adherence (reduced 2.3 to 99%) and invasion (reduced 71.3 to 99.2%) to the HeLa cells. The expression of and was reduced from 66 to 99% and from 81 to 99%, respectively. The total phenolic content of the byproducts varied from 92 to 26 mg GAE/g and the total flavonoids varied from 161 to 29.29 mg QE/g. showed the highest percentage of radical scavenging activity (RSA, 90.1). These extracts can prove as effective alternatives for devising new strategies to control infections.
黏附和侵袭细胞是（）感染发展过程中的关键步骤。在本研究中，将[具体名称1]、[具体名称2]和[具体名称3]的副产物提取物添加到[具体细胞名称]培养物中，并分析了[具体病原体名称]对HeLa细胞的黏附和侵袭以及[相关基因1]和[相关基因2]基因的表达。通过定量逆转录PCR（qRT-PCR）测定基因的相对表达。使用分光光度法测定抗氧化活性。亚抑制浓度的副产物提取物影响对HeLa细胞的黏附（降低2.3%至99%）和侵袭（降低71.3%至99.2%）。[相关基因1]和[相关基因2]的表达分别从66%降低至99%和从81%降低至99%。副产物的总酚含量在92至26 mg GAE/g之间变化，总黄酮含量在161至29.29 mg QE/g之间变化。[具体提取物名称]表现出最高百分比的自由基清除活性（RSA，90.1）。这些提取物可证明是设计控制[具体病原体名称]感染新策略的有效替代物。
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