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土耳其一家研究医院血液恶性肿瘤患者分离的万古霉素耐药粪肠球菌菌株的分子流行病学研究。

Molecular epidemiology of vancomycin-resistant Enterococcus faecium strains isolated from haematological malignancy patients in a research hospital in Turkey.

机构信息

Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Adnan Menderes University, Aydin, Turkey.

Microbiology and Clinical Microbiology Laboratory, Ataturk Training and Research Hospital, Izmir, Turkey.

出版信息

J Med Microbiol. 2010 Jun;59(Pt 6):660-664. doi: 10.1099/jmm.0.012625-0. Epub 2010 Mar 11.

Abstract

Infections and outbreaks of vancomycin-resistant enterococci (VRE) still appear to be rare in Turkey. In the present study, VRE strains isolated during an outbreak in a haematology unit of a training and research hospital in Turkey were typed and their antimicrobial-resistance patterns were characterized by molecular methods. Twelve vancomycin-resistant Enterococcus faecium strains isolated from patients with haematological malignancies were investigated by PCR for the presence of genes encoding resistance to vancomycin, tetracycline, chloramphenicol, gentamicin and erythromycin. Their clonal relationship was evaluated by PFGE and multilocus sequence typing. All strains were resistant to vancomycin and erythromycin, and had the vanA and ermB genes, respectively. PFGE was used to determine the presence of two pulsotypes and determine their subtypes. Pulsotype A belonged to sequence type (ST) 17 and pulsotype B belonged to ST 78. All strains with the vanA gene were not the same clone, indicating multiple acquisitions of resistant isolates, even over such a short time period.

摘要

在土耳其,耐万古霉素肠球菌(VRE)的感染和爆发似乎仍然很少见。在本研究中,通过分子方法对土耳其一家培训和研究医院血液科爆发期间分离的 VRE 菌株进行了分型,并对其抗菌药物耐药模式进行了特征描述。对 12 株来自血液恶性肿瘤患者的耐万古霉素粪肠球菌进行 PCR 检测,以检测编码对万古霉素、四环素、氯霉素、庆大霉素和红霉素耐药的基因。通过 PFGE 和多位点序列分型评估其克隆关系。所有菌株均对万古霉素和红霉素耐药,分别携带 vanA 和 ermB 基因。PFGE 用于确定存在两种脉冲类型并确定其亚型。脉冲型 A 属于序列型(ST)17,脉冲型 B 属于 ST78。所有携带 vanA 基因的菌株均不是同一克隆,表明在如此短的时间内,耐药分离株的多次获得。

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