Department of Pharmaceutical Microbiology of the Jagiellonian University Medical College, Cracow, Poland.
J Physiol Pharmacol. 2009 Dec;60 Suppl 6:33-9.
Candida spp. were found in the gastric mucosa of 27 (17%) patients, out of whom 18 (11%) showed co-existence of the fungi with H. pylori. Analysis of relationship between selected disorders of the upper gastrointestinal tract (non ulcer dyspepsia NUD, gastric ulcer, duodenal ulcer) and infection with H. pylori and/or Candida revealed a link between co-existence of H. pylori with Candida and gastric ulcers suggesting synergism of those microorganism in pathogenesis of the disease. On the contrary, according to quantitative studies performed, the fungi alone do not play a significant role in pathogenesis of the above mentioned disorders as they colonize only epithelium to the extent that is not pathologically significant (<10(3) CFU/ml). Genetical study was carried out on 57 Helicobacter pylori strains isolated from bioptates of the gastric mucosa. The genotypes of the strains (gene cagA and alleles of gene vacA - m1, m2, s1, s2) were determined using the PCR technique. As it was shown, the patients infected with H. pylori strains of genotype cagA+, vacA s1 are exposed to higher risk of peptic ulcer disease (PUD) as compared to the patients infected with cagA-, vacA s2 strains. In the case of the NUD patients a correlation with allele m2 was found only (p<0.001). This may suggest that in future some of the NUD patients infected with cagA+, vacA s1 strains will fall into the group at higher risk for PUD.
27 名（17%）患者的胃黏膜中发现了念珠菌属 spp.，其中 18 名（11%）患者的真菌与 H. pylori 共存。分析上消化道（非溃疡性消化不良 NUD、胃溃疡、十二指肠溃疡）的某些疾病与 H. pylori 和/或念珠菌感染之间的关系，发现 H. pylori 与念珠菌共存与胃溃疡之间存在关联，提示这些微生物在疾病发病机制中存在协同作用。相反，根据进行的定量研究，真菌本身在上述疾病的发病机制中没有发挥重要作用，因为它们仅定植于上皮组织，程度上无病理意义（<10(3)CFU/ml）。对从胃黏膜活检中分离的 57 株幽门螺杆菌（Helicobacter pylori）菌株进行了遗传学研究。使用 PCR 技术确定了菌株的基因型（基因 cagA 和基因 vacA 的等位基因 - m1、m2、s1、s2）。结果表明，与感染 cagA-、vacA s2 菌株的患者相比，感染 cagA+、vacA s1 菌株的患者发生消化性溃疡病（PUD）的风险更高。在 NUD 患者中，仅发现与等位基因 m2 相关（p<0.001）。这可能表明，未来一些感染 cagA+、vacA s1 菌株的 NUD 患者将归入 PUD 高危人群。
