Purwanti Nunuk, Azlina Ahmad, Karabasil Mileva R, Hasegawa Takahiro, Yao Chenjuan, Akamatsu Tetsuya, Hosoi Kazuo
Department of Molecular Oral Physiology, Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima, Japan.
J Med Invest. 2009;56 Suppl:253-4. doi: 10.2152/jmi.56.253.
Ligation of the main excretory duct (MED) of the mouse submandibular gland (SMG) induced the expression of Sca-1, a stem cell marker. Sca-1 expression increased prominently in almost all of cells in the duct system, except the acinar cells. Sca-1 induction was accompanied with phosphorylated-STAT3 (Y705) elevation, which was localized in the nuclei of all duct cells. Electrophoretic mobility shift assay (EMSA) confirmed the specific binding of STAT3 to the GAS sequence, a biding site of gamma interferon activating site. Present study suggested one of the initial steps of the tissue regeneration after injury includes STAT3 pathway.
结扎小鼠下颌下腺（SMG）的主排泄管（MED）可诱导干细胞标志物Sca-1的表达。Sca-1表达在导管系统中几乎所有细胞中显著增加，但腺泡细胞除外。Sca-1的诱导伴随着磷酸化STAT3（Y705）的升高，其定位于所有导管细胞的细胞核中。电泳迁移率变动分析（EMSA）证实STAT3与GAS序列（γ干扰素激活位点的结合位点）特异性结合。本研究表明损伤后组织再生的初始步骤之一包括STAT3途径。
