Mizobe Kenichi, Kawabe Yoshihiro, Bando Yasuhiko, Sakiyama Koji, Araki Hisao, Amano Osamu
Division of Anatomy, Department of Human Development and Fostering, Meikai University School of Dentistry , 1-1 Keyakidai, Sakado, Saitama 350-0283, Japan ; Division of Oral Rehabilitation, Department of Restorative and Biomaterials Sciences, Meikai University School of Dentistry.
Division of Anatomy, Department of Human Development and Fostering, Meikai University School of Dentistry , 1-1 Keyakidai, Sakado, Saitama 350-0283, Japan.
Acta Histochem Cytochem. 2014;47(6):255-64. doi: 10.1267/ahc.14037. Epub 2014 Nov 21.
Salivary glands repair and regenerate following various types of injuries and surgical procedures. However, the tissue responses induced in the contralateral glands have yet to be elucidated in detail. Hsp27, a member of the heat-shock protein (Hsp) family, is strongly expressed in physiological environments, particularly during development. Hsp27 was previously shown to play a role in the regulation of acinar cell proliferation and differentiation in the rat submandibular gland. The present study performed the following surgical treatments on the right submandibular glands of adult rats: 1) duct ligation followed by unligation after one week; 2) partial sialoadenectomy; and 3) total sialoadenectomy. Immunohistochemistry for Hsp27 and Ki67 was performed in the experimental and normal contralateral glands, and localization was histologically and morphometrically analyzed. The results obtained revealed the localization of Hsp27 to the intercalated duct in the submandibular glands of non-treated rats. The expression of Hsp27 was strongly induced in both the uninjured contralateral control glands as well as treated glands of experimental rats regardless of the surgical procedure performed. The number of Hsp27-immunopositive cells increased rapidly following surgery, and subsequently returned to the same level as that in non-treated rats after 4 weeks. However, no marked changes were observed in the number of Ki67-immunopositive proliferating cells. Therefore, the change in the number of Hsp27-immunopositive cells may have contributed to compensatory hypertrophy. The results of the present study indicate that the expression of Hsp27 in the intercalated duct in the submandibular gland may play a role in the differentiation of acinar cells.
唾液腺在遭受各种类型的损伤和外科手术后能够修复和再生。然而，对侧腺体中诱导产生的组织反应尚未得到详细阐明。热休克蛋白（Hsp）家族成员之一的Hsp27，在生理环境中，尤其是在发育过程中强烈表达。先前研究表明，Hsp27在大鼠下颌下腺腺泡细胞增殖和分化的调节中发挥作用。本研究对成年大鼠的右侧下颌下腺进行了以下手术治疗：1）导管结扎，一周后解除结扎；2）部分唾液腺切除术；3）全唾液腺切除术。对实验侧和正常对侧腺体进行Hsp27和Ki67免疫组织化学检测，并对其定位进行组织学和形态计量学分析。结果显示，在未处理大鼠的下颌下腺中，Hsp27定位于闰管。无论进行何种手术操作，未受伤的对侧对照腺体以及实验大鼠的处理腺体中Hsp27的表达均被强烈诱导。手术后，Hsp27免疫阳性细胞数量迅速增加，随后在4周后恢复到与未处理大鼠相同的水平。然而，Ki67免疫阳性增殖细胞数量未观察到明显变化。因此，Hsp27免疫阳性细胞数量的变化可能促成了代偿性肥大。本研究结果表明，下颌下腺闰管中Hsp27的表达可能在腺泡细胞分化中发挥作用。
