Kulkarni Amod P, Randall Philippa J, Murthy Krishna, Kellaway Lauriston A, Kotwal Girish J
Division of Neuroscience, Department of Human Biology, University of Cape Town, Cape Town South Africa.
Open Biochem J. 2010 Jan 27;4:9-21. doi: 10.2174/1874091X01004010009.
C3 and C3b, the components central to the complement activation, also play a damaging role in several inflammatory disorders. Vaccinia virus complement control protein (VCP) and curcumin (Cur) are natural compounds with different biological origins reported to regulate complement activation. However, both VCP and Cur have not been investigated for their interaction with the third component (C3) prior to it being converted to its activated form (C3b). These two compounds have also not been compared to each other with respect to their interactions with C3 and C3b. Quartz crystal microbalance with dissipation monitoring (QCM-D) is a novel technology used to study the interaction of biomolecules. This technology was applied to characterize the interactions of VCP, Cur and appropriate controls with the key complement components. Cur as well as VCP showed binding to both C3 and to C3b, Cur however bound to C3b to a lesser extent.
补体激活的核心成分C3和C3b在多种炎症性疾病中也发挥着破坏作用。牛痘病毒补体控制蛋白（VCP）和姜黄素（Cur）是具有不同生物学来源的天然化合物，据报道可调节补体激活。然而，在VCP和Cur与第三成分（C3）转化为其活化形式（C3b）之前，尚未对它们与C3的相互作用进行研究。这两种化合物在与C3和C3b的相互作用方面也未进行相互比较。带耗散监测的石英晶体微天平（QCM-D）是一种用于研究生物分子相互作用的新技术。该技术用于表征VCP、Cur和适当对照与关键补体成分的相互作用。Cur以及VCP均显示出与C3和C3b结合，但Cur与C3b的结合程度较小。
