Department of Chemical Engineering, University of Vigo, PO Box 36310, Vigo, Spain.
Bioprocess Biosyst Eng. 2010 Oct;33(8):929-35. doi: 10.1007/s00449-010-0416-8. Epub 2010 Mar 12.
The aim of the present study was to investigate the feasibility of several food-processing wastes as support substrate for lipolytic enzymes production by the fungus Rhizopus oryzae under solid-state conditions. Different experiments were conducted to select the variables that allow obtaining high levels of lipolytic enzyme activity. In particular, the use of inert and non-inert solid materials and lipidic and surfactant compounds was evaluated. It was observed that the addition of Triton X-100 together with barley bran involved lipolytic production values tenfold higher than the cultures exclusively grown on an inert support. In addition, from preliminary thermoinactivation kinetics studies, it was concluded that the strategy proposed in this investigation entails another benefit in terms of resistance of the produced enzymes against thermoinactivation.
本研究旨在探讨几种食品加工废物作为米根霉在固态条件下生产脂肪酶的支持基质的可行性。进行了不同的实验来选择能够获得高脂肪酶活性的变量。特别是，评估了惰性和非惰性固体材料以及脂质和表面活性剂化合物的使用。结果表明，与仅在惰性支持物上生长的培养物相比，添加 Triton X-100 和大麦糠可使脂肪酶产量提高十倍。此外，从初步的热失活动力学研究中得出结论，本研究中提出的策略在产生的酶对热失活的抗性方面具有另一个益处。
