Composting Research Group, Department of Chemical Engineering, Escola d'Enginyeria, Universitat Autònoma de Barcelona, Bellaterra, Cerdanyola del Vallès, 08193 Barcelona, Spain.
Bioresour Technol. 2011 Nov;102(21):10080-4. doi: 10.1016/j.biortech.2011.08.062. Epub 2011 Aug 22.
Lipases were produced by a microbial consortium derived from a mixture of wastewater sludges in a medium containing solid industrial wastes rich in fats, under thermophilic conditions (temperature higher than 45°C for 20 days) in 4.5-L reactors. The lipases were extracted from the solid medium using 100mM Tris-HCl, pH 8.0 and a cationic surfactant agent (cetyltrimethylammonium chloride). Different doses of surfactant and buffer were tested according to a full factorial experimental design. The extracted lipases were most active at 61-65°C and at pH 7.7-9. For the solid samples, the lipolytic activity reached up to 120,000 UA/g of dry matter. These values are considerably higher than those previously reported in literature for solid-state fermentation and highlight the possibility to work with the solid wastes as effective biocatalysts.
脂肪酶由微生物联合体产生，该联合体来源于富含脂肪的固体工业废物和废水污泥的混合物，在 4.5-L 反应器中，在嗜热条件（温度高于 45°C 持续 20 天）下进行培养。脂肪酶使用 100mMTris-HCl，pH8.0 和阳离子表面活性剂（十六烷基三甲基氯化铵）从固体培养基中提取。根据完全析因实验设计，测试了不同剂量的表面活性剂和缓冲液。提取的脂肪酶在 61-65°C 和 pH7.7-9 时最活跃。对于固体样品，脂肪酶活性最高可达 120,000UA/g 干物质。这些值明显高于文献中先前报道的固态发酵的值，并突出了将固体废物用作有效生物催化剂的可能性。
