Clinical & Experimental Optometry Research Lab, Department of Physics (Optometry), School of Sciences, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal.
Graefes Arch Clin Exp Ophthalmol. 2010 Aug;248(8):1159-66. doi: 10.1007/s00417-010-1337-6. Epub 2010 Mar 12.
To compute two new quantitative parameters that directly reflect the level of polymegethism and pleomorphism using data provided by a non-contact specular microscope.
We examined right eyes of 306 voluntaries (102 males, 204 females) whose ages ranged from 6 to 82 years (mean +/- SD, 44 +/- 22 years). Endothelial cell density (ECD), average cell size (ACS), standard error of cells surface (SEM), coefficient of variation in cell size (CV) and hexagonality index (HI) were obtained. In addition, two new indices of polymegethism (POLi) and pleomorphism (PLEi) were derived using weighted linear combinations of data obtained from instrument classification of endothelial cells based on the individual counts of cells by size and number of sides respectively, as provided by the instrument. Values of POLi and PLEi were compared between a group of diabetic patients and a group of age-matched controls.
Average values of POLi and PLEi were 10.47 +/- 3.94 and 8.36 +/- 1.21 respectively. POLi and PLEi display high correlation with SEM (r = 0.911, r = 0.664), ECD (r = -0.997, r = -0.585), ACS (r = 0.997, r = 0.441), ACS (SD) (r = 0.883, r = 0.682), CV (r = 0.301, r = 0.712) and HI (r = -0.437, r = -0.991); all these correlations were highly significant (p < 0.001). POLi and PLEi also showed significant positive correlations with age (r = 0.765, p < 0.001 and r = 0.428, p < 0.001 respectively). POLi was significantly higher in a group of diabetic patients when compared with another group of age-matched controls (p = 0.001).
Two single quantitative parameters of endothelial polymegethism and pleomorphism (POLi and PLEi respectively) have been derived from the data obtained with a commercial non-contact specular microscope. These parameters have been demonstrated to identify differences between the corneal endothelium of diabetic and non-diabetic patients.
使用非接触式共焦显微镜提供的数据，计算两个直接反映多形性和多态性水平的新定量参数。
我们检查了 306 名志愿者（男性 102 名，女性 204 名）的右眼，年龄在 6 至 82 岁之间（平均 +/- 标准差，44 +/- 22 岁）。获得内皮细胞密度（ECD）、平均细胞大小（ACS）、细胞表面标准误差（SEM）、细胞大小变异系数（CV）和六边形指数（HI）。此外，使用基于个体细胞大小和细胞数量的仪器分类获得的数据，通过加权线性组合，分别衍生出两个新的多形性（POLi）和多态性（PLEi）指数。将糖尿病患者组和年龄匹配对照组的 POLi 和 PLEi 值进行比较。
POLi 和 PLEi 的平均值分别为 10.47 +/- 3.94 和 8.36 +/- 1.21。POLi 和 PLEi 与 SEM（r = 0.911，r = 0.664）、ECD（r = -0.997，r = -0.585）、ACS（r = 0.997，r = 0.441）、ACS（SD）（r = 0.883，r = 0.682）、CV（r = 0.301，r = 0.712）和 HI（r = -0.437，r = -0.991）高度相关；所有这些相关性均具有统计学意义（p < 0.001）。POLi 和 PLEi 与年龄也呈显著正相关（r = 0.765，p < 0.001 和 r = 0.428，p < 0.001）。与另一组年龄匹配的对照组相比，糖尿病患者组的 POLi 明显更高（p = 0.001）。
从商业非接触式共焦显微镜获得的数据中，分别得出内皮细胞多形性和多态性的两个单一定量参数（分别为 POLi 和 PLEi）。这些参数已被证明可识别糖尿病和非糖尿病患者角膜内皮之间的差异。
