一种用于血管紧张素转换酶的简易放射分析方法。
A simple radioassay for angiotensin-converting enzyme.
作者信息
Ryan J W, Chung A, Ammons C, Carlton M L
出版信息
Biochem J. 1977 Nov 1;167(2):501-4. doi: 10.1042/bj1670501.
Abstract
Angiotensin-converting enzyme can be measured by the rate of release of 3H-labelled hippurate from p-[3H]benzoylglycylglycylglycine. The product is separable from the substrate by extraction of acidified reaction mixtures with ethyl acetate. Assay results for human serum angiotensin-converting enzyme can be obtained within 1.5 h of receipt of serum samples. Within the limits tested, the assay appears to be specific. However, interference by hitherto unrecognized enzymes of abnormal sera must be ruled out.
摘要
血管紧张素转换酶可以通过对 - [³H]苯甲酰甘氨酰甘氨酰甘氨酸中³H标记马尿酸盐的释放速率来测定。通过用乙酸乙酯萃取酸化的反应混合物，可将产物与底物分离。在收到血清样本后1.5小时内即可获得人血清血管紧张素转换酶的检测结果。在所测试的范围内，该检测似乎具有特异性。然而，必须排除异常血清中迄今未被识别的酶的干扰。
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