[X染色体短串联重复序列多重扩增体系的建立与应用]
[Development and application of X-chromosomal STR multiplex amplification system].
作者信息
Liu Qiu-Ling, Lü De-Jian, Zhao Hu
机构信息
Department of Forensic Medicine, Zhongshan Medical College, Sun Yat-sen University, Guangzhou 510080, China.
出版信息
Fa Yi Xue Za Zhi. 2009 Dec;25(6):425-7.
OBJECTIVE
To explore the application of X-chromosomal STR(X-STR) for forensic identification and paternity testing.
METHODS
Six X-STR loci DXS6801, DXS9902, DXS6809, DXS6803, DXS6804 and DXS6799 were amplified in a single PCR reaction. PCR products were analyzed using capillary electrophoresis and 3100 Genetic Analyzer and GeneMapper ID v3.1 Analysis Software.
RESULTS
The alleles of all six X-STR loci were successfully obtained with unambiguous genotyping, high sensitivity and reproducibility.
CONCLUSION
The multiplex PCR of six X-STR loci is useful in forensic identification, particularly for sisters cases.
目的
探讨X染色体短串联重复序列（X-STR）在法医鉴定和亲子鉴定中的应用。
方法
在单个聚合酶链反应（PCR）中扩增6个X-STR基因座DXS6801、DXS9902、DXS6809、DXS6803、DXS6804和DXS6799。使用毛细管电泳、3100基因分析仪和GeneMapper ID v3.1分析软件对PCR产物进行分析。
结果
成功获得了所有6个X-STR基因座的等位基因，基因分型明确，灵敏度高且可重复性强。
结论
6个X-STR基因座的多重PCR在法医鉴定中有用，尤其适用于姐妹案件。
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