[中国珞巴族群体X染色体8个短串联重复序列位点的遗传多态性分析]
[Genetic polymorphism analysis of 8 short tandem repeat loci on the X chromosome in Luoba population of China].
作者信息
Kang Long-li, Ma Yu-ming, Gao Fang, Yu Bing, Li Sheng-bin
机构信息
Life Science Base Laboratory of Tibet National College, Xianyang, Shaanxi, 712082 P. R. China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2007 Aug;24(4):479-81.
OBJECTIVE
To investigate the alleles and genotype frequency of 8 short tandem repeat (STR) loci (DXS6804, DXS8378, DXS7132, DXS7133, DXS6789, DXS101, HPRTB and DXS7423) on the X chromosome in Luoba ethnic group of China.
METHODS
The eight X chromosome STR (X-STR) loci were analyzed by polymerase chain reaction (PCR) followed by polyacylamide gel electrophoresis and silver stain.
RESULTS
Among 96 unrelated Luoba individuals, the numbers of alleles in the 8 X-STR loci (DXS7133, DXS6789, DXS6804, DXS8378, DXS101, DXS7424, DXS7132 and HPRTB) were 5, 8, 7, 5, 8, 8, 8 and 5, respectively. The genotype frequencies in females were in accordance with Hardy-Weinberg equilibrium.
CONCLUSION
The eight X-STR loci are appropriate for individual identification, paternity testing involving a female child and studies on related diseases.
目的
研究中国珞巴族群体X染色体上8个短串联重复序列（STR）基因座（DXS6804、DXS8378、DXS7132、DXS7133、DXS6789、DXS101、HPRTB和DXS7423）的等位基因及基因型频率。
方法
采用聚合酶链反应（PCR）扩增后聚丙烯酰胺凝胶电泳及银染法对8个X染色体STR（X-STR）基因座进行分析。
结果
在96名无血缘关系的珞巴族个体中，8个X-STR基因座（DXS7133、DXS6789、DXS6804、DXS8378、DXS101、DXS7424、DXS7132和HPRTB）的等位基因数分别为5、8、7、5、8、8、8和5。女性基因型频率符合Hardy-Weinberg平衡。
结论
这8个X-STR基因座适用于个体识别、涉及女性子代的亲子鉴定及相关疾病研究。
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