一种包含12个X染色体短串联重复序列的新型多重PCR检测方法的开发与法医学验证
Development and forensic validation of a new multiplex PCR assay with 12 X-chromosomal short tandem repeats.
作者信息
Turrina Stefania, Atzei Renzo, Filippini Giulia, De Leo Domenico
机构信息
Department of Medicine and Public Health, Institute of Legal Medicine, Forensic Genetic Laboratory, University of Verona, Policlinico G.B. Rossi, 37134 Verona, Italy.
出版信息
Forensic Sci Int Genet. 2007 Jun;1(2):201-4. doi: 10.1016/j.fsigen.2007.01.011. Epub 2007 Feb 26.
One multiplex system for the co-amplification of 12 X-chromosomal short tandem repeats (STRs) DXS7132, DXS8378, DXS6809, DXS7133, DXS6789, DXS7424, GATA172D05, HPRTB, DXS7423, GATA31E08, DXS101, DXS6807 and amelogenin was analysed in a sample of 200 (100 males and 100 females) unrelated healthy individuals living in Northern Italy. The chi2-test for genotype distribution of the X-chromosomal STRs showed no significant deviation from the Hardy-Weinberg equilibrium (HWE). Allele frequencies between female and male samples were not significantly different in all examined markers. In the kinship cases involving 40 family trios with daughter and 10 father/daughter duos, no mutation was detected. The combined power of discrimination (PDc) of the 12 X-STRs for both females and males was PDc > 0.999999.
在居住于意大利北部的200名（100名男性和100名女性）无亲缘关系的健康个体样本中，分析了一种用于共同扩增12个X染色体短串联重复序列（STR）DXS7132、DXS8378、DXS6809、DXS7133、DXS6789、DXS7424、GATA172D05、HPRTB、DXS7423、GATA31E08、DXS101、DXS6807和牙釉蛋白的多重系统。对X染色体STR的基因型分布进行的卡方检验显示，与哈迪-温伯格平衡（HWE）无显著偏差。在所有检测的标记中，女性和男性样本之间的等位基因频率无显著差异。在涉及40个有女儿的家庭三联体和10个父女二人组的亲属关系案例中，未检测到突变。12个X-STR对女性和男性的联合鉴别力（PDc）为PDc>0.999999。
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