Department of Medicine, Division of Pulmonary Medicine, University of Helsinki, Helsinki, Finland.
BMC Pulm Med. 2010 Mar 14;10:13. doi: 10.1186/1471-2466-10-13.
Smoking cessation is the best possible way to prevent the progression of smoking related airway diseases. However, the effect and time scale of smoking cessation on airway inflammation/remodelling are largely unknown. This prospective study evaluated the effects of smoking cessation on induced sputum (IS) neutrophils, matrix metalloproteinases (MMP-7, -8, -9) and tissue inhibitor of metalloproteinase-1 (TIMP-1).
A total of 61 subjects participated in the study; 17 stopped smoking for 3 months and 9 for 6 months. The proportion of IS neutrophils and the levels of MMPs and TIMP-1 by ELISA were determined at baseline and at 3 and 6 months after cessation.
In the smokers, baseline IS neutrophils, MMPs and TIMP-1 were significantly higher compared to non-smokers. Levels of MMP-7, -8 and TIMP-1 decreased nearly to those of non-smokers but the levels of MMP-9 increased significantly from the baseline of the same subjects at 3 months after cessation (p = 0.009) with no significant decline at 6 months after cessation.
Sputum MMP-9 remained elevated after 6 months of smoking cessation, which may contribute to ongoing lung damage typical of COPD.
戒烟是预防与吸烟相关的气道疾病进展的最佳方法。然而，戒烟对气道炎症/重塑的效果和时间尺度在很大程度上尚不清楚。本前瞻性研究评估了戒烟对诱导痰（IS）中性粒细胞、基质金属蛋白酶（MMP-7、-8、-9）和金属蛋白酶组织抑制剂-1（TIMP-1）的影响。
共有 61 名受试者参与了这项研究；17 名受试者戒烟 3 个月，9 名受试者戒烟 6 个月。在基线和戒烟后 3 个月和 6 个月时，通过 ELISA 测定 IS 中性粒细胞的比例以及 MMPs 和 TIMP-1 的水平。
在吸烟者中，IS 中性粒细胞、MMPs 和 TIMP-1 的基线水平明显高于不吸烟者。MMP-7、-8 和 TIMP-1 的水平几乎降至不吸烟者的水平，但 MMP-9 的水平在戒烟后 3 个月时从同一受试者的基线水平显著升高（p = 0.009），在戒烟后 6 个月时没有显著下降。
戒烟 6 个月后，痰 MMP-9 仍保持升高，这可能导致 COPD 典型的持续肺损伤。
