Department of Obstetrics and Gynecology, Shanghai Sixth People's Hospital, Shanghai Jiaotong University, Shanghai, 200233 China.
Thromb Res. 2010 Jul;126(1):e41-5. doi: 10.1016/j.thromres.2010.02.012. Epub 2010 Mar 11.
To investigate the mechanism underlying the hypercoagulable state in severe pre-eclampsia.
Plasma tissue factor (TF) and tissue factor pathway inhibitor (TFPI) expression from pre-eclampsia patients and healthy pregnant controls were determined by ELISA. Placental TF and TFPI gene and protein expression were detected by quantitative RT-PCR, immunohistochemistry, and Western analysis.
The plasma TF level in the pre-eclampsia group was significantly higher than the control group (p<0.01), and surprisingly, the plasma TFPI-1 and TFPI-2 in the pre-eclampsia group were significantly lower (p<0.01). Placental TF gene and protein expression levels in the pre-eclampsia group was significantly higher than the control group, while TFPI-1 and TFPI-2 levels were significantly lower (p<0.05). Lastly, a significant correlation was found between plasma and placental TF protein levels in the pre-eclampsia group (p<0.01).
Higher expression and/or release of TF from the placenta may contribute towards a pathological hypercoagulable state in pre-eclampsia patients.
探讨重度子痫前期高凝状态的发生机制。
采用 ELISA 法检测子痫前期患者和健康孕妇对照者的血浆组织因子（TF）和组织因子途径抑制剂（TFPI）表达。采用定量 RT-PCR、免疫组织化学和 Western 分析检测胎盘 TF 和 TFPI 基因和蛋白表达。
子痫前期组血浆 TF 水平明显高于对照组（p<0.01），令人惊讶的是，子痫前期组血浆 TFPI-1 和 TFPI-2 明显降低（p<0.01）。子痫前期组胎盘 TF 基因和蛋白表达水平明显高于对照组，而 TFPI-1 和 TFPI-2 水平明显降低（p<0.05）。最后，子痫前期组血浆和胎盘 TF 蛋白水平之间存在显著相关性（p<0.01）。
胎盘 TF 的高表达和/或释放可能导致子痫前期患者发生病理性高凝状态。
