严重子痫前期患者血浆与胎盘组织因子及组织因子途径抑制剂的关系。
The relationship between plasma and placental tissue factor, and tissue factor pathway inhibitors in severe pre-eclampsia patients.
机构信息
Department of Obstetrics and Gynecology, Shanghai Sixth People's Hospital, Shanghai Jiaotong University, Shanghai, 200233 China.
出版信息
Thromb Res. 2010 Jul;126(1):e41-5. doi: 10.1016/j.thromres.2010.02.012. Epub 2010 Mar 11.
OBJECTIVE
To investigate the mechanism underlying the hypercoagulable state in severe pre-eclampsia.
METHODS
Plasma tissue factor (TF) and tissue factor pathway inhibitor (TFPI) expression from pre-eclampsia patients and healthy pregnant controls were determined by ELISA. Placental TF and TFPI gene and protein expression were detected by quantitative RT-PCR, immunohistochemistry, and Western analysis.
RESULTS
The plasma TF level in the pre-eclampsia group was significantly higher than the control group (p<0.01), and surprisingly, the plasma TFPI-1 and TFPI-2 in the pre-eclampsia group were significantly lower (p<0.01). Placental TF gene and protein expression levels in the pre-eclampsia group was significantly higher than the control group, while TFPI-1 and TFPI-2 levels were significantly lower (p<0.05). Lastly, a significant correlation was found between plasma and placental TF protein levels in the pre-eclampsia group (p<0.01).
CONCLUSION
Higher expression and/or release of TF from the placenta may contribute towards a pathological hypercoagulable state in pre-eclampsia patients.
目的
探讨重度子痫前期高凝状态的发生机制。
方法
采用 ELISA 法检测子痫前期患者和健康孕妇对照者的血浆组织因子（TF）和组织因子途径抑制剂（TFPI）表达。采用定量 RT-PCR、免疫组织化学和 Western 分析检测胎盘 TF 和 TFPI 基因和蛋白表达。
结果
子痫前期组血浆 TF 水平明显高于对照组（p<0.01），令人惊讶的是，子痫前期组血浆 TFPI-1 和 TFPI-2 明显降低（p<0.01）。子痫前期组胎盘 TF 基因和蛋白表达水平明显高于对照组，而 TFPI-1 和 TFPI-2 水平明显降低（p<0.05）。最后，子痫前期组血浆和胎盘 TF 蛋白水平之间存在显著相关性（p<0.01）。
结论
胎盘 TF 的高表达和/或释放可能导致子痫前期患者发生病理性高凝状态。
Zotero 插件