Obstetrics & Gynecology Hospital, Fudan University, China.
Thromb Res. 2010 Jun;125(6):e317-22. doi: 10.1016/j.thromres.2010.02.017. Epub 2010 Mar 29.
To investigate the maternal and fetal plasma changes of tissue factor pathway inibitor-2 (TFPI-2) and its placental expression in women with normal pregnancy and preeclampsia.
We assessed the plasma TFPI-2 level in non-pregnant, normal pregnant and postpartum women, detected fetal plasma level and expression in placenta, and compared the changes in women with preeclampsia. Time-resolved fluoroimmunoassay and immunohistochemistry were used for plasma and placenta tissue detection, respectively.
Maternal plasma levels of TFPI-2 in normal pregnant women at 13weeks of gestation increased 9.3 times as compared with healthy non-pregnant women (149.3+/-17.1 versus 16.0+/-3.6ng/ml)reached a maximum level ( 282.6+/-17.1ng/ml) at 39weeks of gestation, and dramatically decreased to nearly a non-pregnant level on the first day of postpartum (32.3+/-7.1ng/ml); similar change was found in the placental expression. Fetal plasma TFPI-2 was significantly lower than the maternal level at delivery. The maternal plasma TFPI-2 in preeclampsia was significantly lower compared with that in normal pregnancy, coupled with significantly higher placental expression.
Placenta may be the main site of the high level of TFPI-2 production in maternal circulation, and the dramatic changes in preeclampsia provide a clue to elucidate its pathogenesis.
研究组织因子途径抑制物-2（TFPI-2）在正常妊娠和子痫前期孕妇母血和胎盘中的变化及其胎盘表达。
我们评估了非妊娠、正常妊娠和产后妇女的血浆 TFPI-2 水平，检测了胎儿血浆水平和胎盘表达，并比较了子痫前期妇女的变化。时间分辨荧光免疫分析法和免疫组织化学法分别用于血浆和胎盘组织检测。
正常妊娠妇女 13 周时母体血浆 TFPI-2 水平较健康非妊娠妇女增加 9.3 倍（149.3±17.1 与 16.0±3.6ng/ml），在 39 周时达到最高水平（282.6±17.1ng/ml），产后第一天迅速降至接近非妊娠水平（32.3±7.1ng/ml）；胎盘表达也有类似变化。胎盘中 TFPI-2 的表达在分娩时明显低于母体水平。子痫前期患者的母体血浆 TFPI-2 明显低于正常妊娠，同时胎盘表达明显升高。
胎盘可能是母体循环中 TFPI-2 高水平产生的主要部位，子痫前期的显著变化为阐明其发病机制提供了线索。
