de los Santos E T, Mazzaferri E L
Department of Internal Medicine, Ohio State University, Columbus 43210.
J Clin Endocrinol Metab. 1991 May;72(5):1031-5. doi: 10.1210/jcem-72-5-1031.
Plasma immunoreactive calcitonin-gene related peptide (CGRP) concentrations were measured in 10 normal male subjects, aged 23-34 yr, who were studied under 3 separate conditions at intervals of at least 1 week. In phase 1, plasma CGRP concentrations were measured hourly for 24 h during normal activity; in phase 2, the CGRP response to diuretic-induced volume depletion and upright posture was measured over 3 h; in phase 3, the CGRP response to volume loading and supine posture was measured over 4 h. During phase 1, the mean 24-h plasma CGRP concentration averaged 30 +/- 16 pmol/L; hourly CGRP concentrations ranged from 25-37 pmol/L over 24 h and oscillated from 16% below to 27% above the mean 24-h CGRP value, changes that were highly significant (P = 0.005). The peaks were at 0600 and 0700 h, while the nadirs were at 1200 and 2000 h. No significant difference was found between fasting CGRP at 0800 h and levels taken 1, 2, and 3 h postprandially (P = 0.68). During phase 2 (volume depletion and upright) mean plasma CGRP rose significantly, going from a baseline of 42 +/- 8 pmol/L at 0800 h to a peak of 60 +/- 10 pmol/L at 1100 h (P = 0.0005). The percent CGRP increment was relatively small (26% to 42%) compared to the rise in PRA (518% to 1033%) and aldosterone concentrations (333% to 465%). Hematocrit increased from 40.5 +/- 0.7% to 45.8 +/- 0.7% (P = 0.0001) in phase 2, while serum osmolality did not change significantly. In phase 3 (volume loading and supine), plasma CGRP did not change significantly, while PRA and aldosterone concentrations fell significantly (P = 0.0001); neither serum osmolality nor hematocrit changed significantly. We conclude that plasma CGRP concentrations fluctuate spontaneously and significantly during the day, and rise in response to volume depletion. The reasons for the CGRP fluctuations seen over 24 h, which are not apparent from this study, may be related to changes in vascular volume or other as yet unidentified factors. These studies are consistent with the concept that CGRP, acting either directly or through the renin-aldosterone system, may have a role in regulating peripheral vascular tone or controlling vascular volume.
对10名年龄在23 - 34岁的正常男性受试者进行了血浆免疫反应性降钙素基因相关肽（CGRP）浓度的测定，这些受试者在至少间隔1周的3种不同条件下接受研究。在第1阶段，在正常活动期间每小时测量血浆CGRP浓度，持续24小时；在第2阶段，在3小时内测量CGRP对利尿剂诱导的容量减少和直立姿势的反应；在第3阶段，在4小时内测量CGRP对容量负荷和仰卧姿势的反应。在第1阶段，24小时血浆CGRP平均浓度为30±16 pmol/L；24小时内每小时CGRP浓度范围为25 - 37 pmol/L，在24小时CGRP平均值上下波动16%至27%，这些变化具有高度显著性（P = 0.005）。峰值出现在06:00和07:00，而谷值出现在12:00和20:00。08:00的空腹CGRP与餐后1、2和3小时的水平之间未发现显著差异（P = 0.68）。在第2阶段（容量减少和直立），血浆CGRP显著升高，从08:00的基线42±8 pmol/L升至11:00的峰值60±10 pmol/L（P = 0.0005）。与肾素活性（PRA）升高（518%至1033%）和醛固酮浓度升高（333%至465%）相比，CGRP升高的百分比相对较小（26%至42%）。在第2阶段，血细胞比容从40.5±0.7%增加到45.8±0.7%（P = 0.0001），而血清渗透压没有显著变化。在第3阶段（容量负荷和仰卧），血浆CGRP没有显著变化，而PRA和醛固酮浓度显著下降（P = 0.0001）；血清渗透压和血细胞比容均无显著变化。我们得出结论，血浆CGRP浓度在一天中会自发且显著波动，并对容量减少产生反应而升高。24小时内观察到的CGRP波动原因，本研究未明确，可能与血管容量变化或其他尚未确定的因素有关。这些研究与CGRP可能直接或通过肾素 - 醛固酮系统在调节外周血管张力或控制血管容量中起作用的概念一致。
