Department of Molecular Biotechnology, Ghent University, Coupure Links 653, 9000 Ghent, Belgium.
Vet Rec. 2010 Mar 13;166(11):329-33. doi: 10.1136/vr.b4714.
A concurrent outbreak of chlamydial disease in boars, sows and gilts and postweaning multisystemic wasting syndrome (PMWS) in weaned piglets was investigated on a large pig production farm in Estonia. Chlamydia suis DNA was detected in conjunctival swabs from boars, sows and gilts, but also in the faeces of boars and sows. Chlamydophila abortus DNA was found in semen, and in conjunctival swabs from sows; DNA was demonstrated by microarrays. Serum samples from boars were examined using a Chlamydiaceae-specific recombinant ELISA. All 10 serum samples examined were positive (1:960 to 1:3840). Chlamydiosis was characterised by reproductive failure and conjunctivitis. Piglets were not examined for Chlamydiaceae, as eye problems were not observed. Piglets showed wasting, respiratory signs, diarrhoea, enlargement of lymph nodes and increased mortality (10 per cent). Porcine circovirus type 2 (PCV-2) was detected in the lymph nodes of piglets by immunohistochemistry, and PCV-2 antibodies were demonstrated in all 10 serum samples from sows examined using an immunoperoxidase monolayer assay.
在爱沙尼亚的一个大型养猪场，对种猪、母猪和后备母猪中的衣原体病以及断奶仔猪的多系统衰弱综合征（PMWS）的并发疫情进行了调查。在种猪、母猪和后备母猪的眼结膜拭子中，以及在种猪的粪便中检测到了猪衣原体 DNA。在精液和母猪的眼结膜拭子中发现了衣原体 DNA；微阵列证实了这一点。使用针对衣原体科的重组 ELISA 检测了种猪的血清样本。检查的 10 份血清样本均为阳性（1:960 至 1:3840）。衣原体病的特征是繁殖失败和结膜炎。由于未观察到眼部问题，因此未对仔猪进行衣原体检查。仔猪表现出消瘦、呼吸症状、腹泻、淋巴结肿大和死亡率增加（10%）。通过免疫组织化学在仔猪的淋巴结中检测到了猪圆环病毒 2 型（PCV-2），并使用免疫过氧化物酶单层试验检测了所有 10 份来自母猪的血清样本中的 PCV-2 抗体。
