肽酶联免疫吸附测定法（ELISA）和荧光共振能量转移定量聚合酶链反应（FRET-qPCR）结果表明，美国亚拉巴马州家猪体内某物质的流行率显著高于野猪。

Peptide ELISA and FRET-qPCR Identified a Significantly Higher Prevalence of in Domestic Pigs Than in Feral Swine from the State of Alabama, USA.

作者信息

Hoque Md Monirul, Adekanmbi Folasade, Barua Subarna, Rahman Kh Shamsur, Aida Virginia, Anderson Brian, Poudel Anil, Kalalah Anwar, Bolds Sara, Madere Steven, Kitchens Steven, Price Stuart, Brown Vienna, Lockaby B Graeme, Kyriakis Constantinos S, Kaltenboeck Bernhard, Wang Chengming

机构信息

College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA.

Swine Research and Education Center, Auburn University, Auburn, AL 36830, USA.

出版信息

Pathogens. 2020 Dec 25;10(1):11. doi: 10.3390/pathogens10010011.

DOI:10.3390/pathogens10010011

PMID:33375583

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7823902/

Abstract

is an important, highly prevalent, and diverse obligate intracellular pathogen infecting pigs. In order to investigate the prevalence and diversity of in the U.S., 276 whole blood samples from feral swine were collected as well as 109 fecal swabs and 60 whole blood samples from domestic pigs. -specific peptide ELISA identified anti- antibodies in 13.0% of the blood of feral swine (26/276) and 80.0% of the domestic pigs (48/60). FRET-qPCR and DNA sequencing found DNA in 99.1% of the fecal swabs (108/109) and 21.7% of the whole blood (13/60) of the domestic pigs, but not in any of the assayed blood samples (0/267) in feral swine. Phylogenetic comparison of partial ompA gene sequences and -specific multilocus sequencing typing (MLST) revealed significant genetic diversity of the identified in this study. Highly genetically diverse strains are prevalent in domestic pigs in the USA. As crowding strongly enhances the frequency and intensity of highly prevalent infections in animals, less population density in feral swine than in domestic pigs may explain the significantly lower prevalence in feral swine. A future study is warranted to obtain DNA from feral swine to perform genetic diversity of between commercial and feral pigs.

摘要

是一种重要的、高度流行且多样的专性细胞内病原体，可感染猪。为了调查美国的流行情况和多样性，采集了276份野猪全血样本以及109份家猪粪便拭子和60份家猪全血样本。特异性肽ELISA在13.0%的野猪血液（26/276）和80.0%的家猪血液（48/60）中检测到抗抗体。荧光定量PCR和DNA测序在家猪99.1%的粪便拭子（108/109）和21.7%的全血（13/60）中发现了DNA，但在野猪的任何检测血液样本（0/267）中均未发现。对部分ompA基因序列进行系统发育比较和特异性多位点测序分型（MLST），揭示了本研究中鉴定出的的显著遗传多样性。遗传高度多样的菌株在美国家猪中普遍存在。由于拥挤会强烈增加动物中高度流行感染的频率和强度，野猪的种群密度低于家猪可能解释了野猪中流行率显著较低的原因。有必要开展进一步研究，从野猪中获取DNA，以研究商业猪和野猪之间的遗传多样性。