Schable B, Villarino M E, Favero M S, Miller J M
Nosocomial Infections Laboratory Branch, Centers for Disease Control, U.S. Dept. of Health and Human Services, Atlanta, Georgia 30333.
Infect Control Hosp Epidemiol. 1991 Mar;12(3):163-7. doi: 10.1086/646310.
To test the utility of a newly developed multilocus enzyme electrophoresis typing method for Xanthomonas maltophilia.
Isolates were first screened by slide agglutination, which served as the standard to characterize the outbreak strains. All isolates were then subjected to multilocus enzyme electrophoresis and the results analyzed based on epidemiological data.
This outbreak occurred in a shock-trauma intensive care unit of a large general community hospital.
Patients admitted to the shock-trauma intensive care unit who had X maltophilia isolated from any site greater than or equal to 24 hours after admission met the case definition. Specimens from patients who fit the case definition were characterized, as were specimens from other patients that were used as controls for nonoutbreak isolates. Environmental samples were also evaluated for X maltophilia.
Most of the 64 isolates received during this outbreak were serotype 10, and when they were subjected to multilocus enzyme electrophoresis, one electrophoretic type predominated and correlated to most outbreak isolates. Unrelated isolates of serotype 10 from other institutions all exhibited unique electrophoretic types.
Application of multilocus enzyme electrophoresis to X maltophilia outbreaks is a valuable addition to the characterization of suspected outbreak strains.
检测一种新开发的嗜麦芽窄食单胞菌多位点酶电泳分型方法的实用性。
首先通过玻片凝集法对分离株进行筛查，以此作为鉴定暴发菌株特征的标准。然后对所有分离株进行多位点酶电泳，并根据流行病学数据对结果进行分析。
此次暴发发生在一家大型综合社区医院的休克创伤重症监护病房。
入住休克创伤重症监护病房且在入院后24小时或更长时间从任何部位分离出嗜麦芽窄食单胞菌的患者符合病例定义。对符合病例定义的患者的标本进行特征分析，同时也对作为非暴发分离株对照的其他患者的标本进行特征分析。还对环境样本进行了嗜麦芽窄食单胞菌的评估。
此次暴发期间收到的64株分离株大多为血清型10，对其进行多位点酶电泳时，一种电泳型占主导，且与大多数暴发分离株相关。来自其他机构的血清型10无关分离株均表现出独特的电泳型。
将多位点酶电泳应用于嗜麦芽窄食单胞菌暴发，是对疑似暴发菌株进行特征分析的一项有价值的补充。
