Orr K, Gould F K, Sisson P R, Lightfoot N F, Freeman R, Burdess D
Department of Microbiology, Freeman Hospital, Newcastle upon Tyne.
J Hosp Infect. 1991 Mar;17(3):187-95. doi: 10.1016/0195-6701(91)90230-6.
Seventeen strains of Xanthomonas maltophilia and one strain of Pseudomonas cepacia were examined by pyrolysis mass spectrometry (PYMS). The Xanthomonas strains comprised 11 clinical and environmental isolates from a suspected outbreak of colonization and infection on a heart-lung transplant intensive care unit, two strains from patients elsewhere in the same hospital and four strains from a national reference collection. The single isolate of Pseudomonas cepacia was from a sink in the same affected intensive care unit. A series of discriminant analyses performed on the PYMS-derived data showed that, whereas six strains of Xanthomonas from the respiratory tract, blood and ventilatory equipment of one of the affected patients were indistinguishable, all the other isolates were distinct. The results of PYMS rapid inter-strain comparison were in accord with those of an epidemiological investigation which suggested that the episode was due to unauthorized reuse of disposable nebulizers and not to cross-infection between patients. Pyrolysis mass spectrometry with rapid data analysis is a potentially useful technique for the investigation of nosocomial infections due to organisms such as X. maltophilia.
采用热解质谱法（PYMS）对17株嗜麦芽窄食单胞菌和1株洋葱伯克霍尔德菌进行了检测。嗜麦芽窄食单胞菌菌株包括11株来自心肺移植重症监护病房疑似定植和感染暴发的临床及环境分离株、2株来自同一家医院其他地方患者的菌株以及4株来自国家参考菌种保藏中心的菌株。洋葱伯克霍尔德菌的单一分离株来自同一受影响重症监护病房的一个水槽。对PYMS衍生数据进行的一系列判别分析表明，虽然来自一名受影响患者呼吸道、血液和通气设备的6株嗜麦芽窄食单胞菌无法区分，但所有其他分离株都是不同的。PYMS快速菌株间比较的结果与流行病学调查结果一致，该调查表明该事件是由于一次性雾化器的未经授权再利用，而非患者之间的交叉感染。具有快速数据分析功能的热解质谱法是一种潜在有用的技术，可用于调查由嗜麦芽窄食单胞菌等微生物引起的医院感染。
