ATTO, Bunkyo-ku, Tokyo 113-0034, Japan.
Anal Biochem. 2010 Jul 1;402(1):107-9. doi: 10.1016/j.ab.2010.03.014. Epub 2010 Mar 15.
We constructed a multichannel perfusion culture system. Using this device, we developed a perfusion device to detect six samples at the same time and demonstrated the response to dexamethasone of the circadian-related promoter activity in Rat1 fibroblast cells. We could detect the sequential phase shifts of the circadian peaks that are dependent on the timing of the drug treatments. We also demonstrate a temporal dual reporter assay using two kinds of secreted luciferase in the perfusion culture. The combination of secreted luciferase and multiple perfusion culture assay system reveals the effects of transient drug treatment for the pharmacological assay.
我们构建了一个多通道灌注培养系统。使用该装置，我们开发了一种灌注设备，可以同时检测六个样本，并展示了 Rat1 成纤维细胞中生物钟相关启动子活性对地塞米松的反应。我们可以检测到生物钟峰值的顺序相位移动，这取决于药物处理的时间。我们还在灌注培养中使用两种分泌型荧光素酶进行了时间双重报告基因检测。分泌型荧光素酶和多灌注培养检测系统的结合揭示了瞬时药物处理对药理学检测的影响。
