Regenerative Medicine/Tissue Engineering Division, Research Center for Cooperative Projects, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
Biotechniques. 2010 Jun;48(6):460-2. doi: 10.2144/000113419.
Bioluminescence imaging reveals the long-term dynamics of individual gene expression in a single cell. However, methods for simultaneously imaging multiple gene expression patterns have been unknown to date. Here, we constructed a dual-path optical luminescence imaging system using a two-color reporter system and could simultaneously track two gene expression patterns for several days in a single cell.
生物发光成像是一种揭示单个细胞内单个基因表达长期动力学的方法。然而，迄今为止，还没有能够同时对多个基因表达模式进行成像的方法。在这里，我们构建了一个使用双色报告基因系统的双通道光学发光成像系统，可以在单个细胞中同时跟踪两种基因表达模式数天。
