进一步的数据表明，在西班牙市场上供应的谷物中存在镰刀菌属真菌产生的真菌毒素恩镰孢菌素、呋塞米和布草菌素。

Further data on the presence of Fusarium emerging mycotoxins enniatins, fusaproliferin and beauvericin in cereals available on the Spanish markets.

机构信息

Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, Burjassot, Valencia, Spain.

出版信息

Food Chem Toxicol. 2010 May;48(5):1412-6. doi: 10.1016/j.fct.2010.03.010. Epub 2010 Mar 15.

DOI:10.1016/j.fct.2010.03.010

PMID:20230871

Abstract

In this work, 64 samples of cereals purchased from local markets in the Valencian community (Spain) were investigated for the presence of six emerging mycotoxins: enniatins ENs (ENA, ENA1, ENB and ENB1), beauvericin (BEA) and fusaproliferin (FUS). Samples were extracted with a mixture of water/acetonitrile (85/15, v/v) by using an Ultra-turrax homogenizer. Mycotoxins were then identified and quantified with a liquid chromatography (LC) with diode array detector (DAD). Positive samples were confirmed with an LC-MS/MS. Analytical Results showed that the frequencies of contamination of samples with ENs, BEA and FUS were 73.4%, 32.8% and 7.8%, respectively. ENA1 was the most mycotoxin found and levels ranged from 33.38 to 814.42 mg/kg. ENB levels ranged between 2.23 and 21.37 mg/kg. ENB1 levels varied from 4.34 to 45.94 mg/kg. All samples were free of ENA. BEA levels ranged from 0.51 to 11.78 mg/kg and FUS levels varied between 1.01 and 6.63 mg/kg. It could be concluded from this study that the high contamination levels found especially for ENs could be of a negative impact on the population. This is the first paper on the presence of emerging mycotoxins in cereals available in Spain.

摘要

在这项工作中，调查了从西班牙巴伦西亚社区当地市场购买的 64 份谷物样本，以检测六种新兴霉菌毒素的存在：恩镰孢菌素（ENA、ENA1、ENB 和 ENB1）、 beauvericin（BEA）和 fusaproliferin（FUS）。使用 Ultra-turrax 匀浆器，用水/乙腈（85/15，v/v）混合物提取样本。然后使用二极管阵列检测器（DAD）的液相色谱（LC）对霉菌毒素进行鉴定和定量。阳性样本用 LC-MS/MS 进行确认。分析结果表明，ENs、BEA 和 FUS 污染样品的频率分别为 73.4%、32.8%和 7.8%。ENA1 是最常见的霉菌毒素，含量范围为 33.38 至 814.42 mg/kg。ENB 水平在 2.23 至 21.37 mg/kg 之间。ENB1 水平在 4.34 至 45.94 mg/kg 之间。所有样品均不含 ENA。BEA 水平在 0.51 至 11.78 mg/kg 之间，FUS 水平在 1.01 至 6.63 mg/kg 之间。从这项研究可以得出结论，尤其是恩镰孢菌素的高污染水平可能会对人群产生负面影响。这是西班牙首次关于谷物中存在新兴霉菌毒素的研究。