College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi, 712100, People's Republic of China.
Mol Biol Rep. 2010 Dec;37(8):3945-9. doi: 10.1007/s11033-010-0052-z. Epub 2010 Mar 16.
The objectives of the present study were focused on detecting deletion mutation in bovine AMPD1 gene, and analyzing its effect on body measurement and carcass traits in Qinchuan cattle by using DNA sequencing and agarose electrophoresis methods. The 198-bp PCR products of AMPD1 gene exhibited three genotypes and two alleles were revealed: A and B. The frequencies of genotype AA/AB/BB in Qinchuan populations was 0.7163, 0.2233 and 0.0605. The χ(2)-test analysis demonstrated that the breed was not in agreement with Hardy-Weinberg equilibrium (P < 0.05). The association of the 18-bp deletion mutation of AMPD1 gene with body measurement and carcass traits of Qinchuan cattle were analyzed. The cattle with AA genotype had slaughter weight and carcass weight than those with genotype AB (P < 0.01 or P < 0.05). These results suggest that the 18-bp deletion mutation may influence the carcass traits in Qinchuan cattle.
本研究的目的是通过 DNA 测序和琼脂糖电泳方法检测牛 AMPD1 基因中的缺失突变，并分析其对秦川牛体尺和胴体性状的影响。AMPD1 基因的 198bp PCR 产物显示出三种基因型和两种等位基因：A 和 B。秦川牛群体中 AA/AB/BB 基因型的频率分别为 0.7163、0.2233 和 0.0605。卡方检验分析表明，该品种与 Hardy-Weinberg 平衡不一致（P < 0.05）。分析了 AMPD1 基因 18bp 缺失突变与秦川牛体尺和胴体性状的关系。AA 基因型牛的屠宰体重和胴体重量均高于 AB 基因型牛（P < 0.01 或 P < 0.05）。这些结果表明，18bp 缺失突变可能影响秦川牛的胴体性状。
