College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China.
College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Cells. 2023 Feb 8;12(4):539. doi: 10.3390/cells12040539.
The () gene regulates limb skeletal development and muscle growth, thus, it was selected as a candidate gene for bovine carcass traits. In this study, we analyzed the mRNA expression level of in various tissues and cells, and determined the genetic variations in the gene, which might be used as molecular markers for cattle breeding. The mRNA expression profiles of in bovine different tissues showed that was highly expressed in both fat and muscle. The gene expression trend of in myoblasts and adipocytes indicated that might be involved in the differentiation of bovine myoblasts and adipocytes. The data in the Ensembl database showed that there are two putative insertion/deletion (InDel) polymorphisms in the bovine gene. The insertion site (rs515880802) was located in the upstream region (NC_037331.1: g. 68853364-68853365) and named as P1-Ins-4-bp, and the deletion site (rs517582703) was located in the intronic region (NC_037331.1: g. 68859510-68859517) and named as P2-Del-8-bp. These polymorphisms within the gene were identified and genotyped by PCR amplification, agarose gel electrophoresis and DNA sequencing in the 640 Shandong Black Cattle Genetic Resource (SDBCGR) population. Moreover, the mutation frequency was very low after detection, so the mathematical expectation (ME) method was used for detection. Statistical analysis demonstrated that P1-Ins-4-bp was significantly correlated with the beef shoulder ( = 0.012) and tongue root ( = 0.004). Meanwhile, P2-Del-8-bp displayed a significant correlation with the back tendon ( = 0.008), money tendon ( = 2.84 × 10), thick flank ( = 0.034), beef shin ( = 9.09 × 10), triangle thick flank ( = 0.04), triangle flank ( = 1.00 × 10), rump ( = 0.018) and small tenderloin ( = 0.043) in the female SDBCGR population. In summary, these outcomes may provide a new perspective for accelerating the molecular breeding of cattle through marker-assisted selection (MAS) strategies.
（）基因调节肢体骨骼发育和肌肉生长，因此被选为牛体尺性状的候选基因。本研究分析了不同组织和细胞中基因的 mRNA 表达水平，确定了基因中的遗传变异，这些变异可能作为牛的分子标记用于育种。牛不同组织中基因的 mRNA 表达谱表明，在脂肪和肌肉中均高度表达。成肌细胞和脂肪细胞中基因表达趋势表明，可能参与牛成肌细胞和脂肪细胞的分化。Ensembl 数据库中的数据显示，牛基因中有两个假定的插入/缺失（InDel）多态性。插入位点（rs515880802）位于上游区域（NC_037331.1：g.68853364-68853365），命名为 P1-Ins-4-bp，缺失位点（rs517582703）位于内含子区域（NC_037331.1：g.68859510-68859517），命名为 P2-Del-8-bp。在 640 头山东黑牛遗传资源（SDBCGR）群体中，通过 PCR 扩增、琼脂糖凝胶电泳和 DNA 测序对这些基因内的多态性进行了鉴定和基因分型。此外，经检测突变频率非常低，因此采用期望均值（ME）法进行检测。统计分析表明，P1-Ins-4-bp 与牛肩（=0.012）和舌根（=0.004）显著相关。同时，P2-Del-8-bp 与背筋（=0.008）、金钱筋（=2.84×10）、厚腰肉（=0.034）、牛腱（=9.09×10）、三角厚腰肉（=0.04）、三角腰肉（=1.00×10）、臀肉（=0.018）和小里脊（=0.043）在雌性 SDBCGR 群体中呈显著相关。总之，这些结果可能为通过标记辅助选择（MAS）策略加速牛的分子育种提供新视角。
