从突尼斯土壤中分离、鉴定和表征一种新的脂肪酶假单胞菌 AHD-1 株。

Isolation, identification and characterization of a new lipolytic pseudomonas sp., strain AHD-1, from Tunisian soil.

机构信息

Laboratoire des Bioprocédés, Pôle d'Excellence Régionale A UF, (PER-LBP), Centre de Biotechnologie de Sfax, Sfax, Tunisia.

出版信息

Environ Technol. 2010 Jan;31(1):87-95. doi: 10.1080/09593330903369994.

DOI:10.1080/09593330903369994

PMID:20232682

Abstract

A novel, lipid-degrading bacterium (strain AHD-1) was isolated from soil regularly contaminated with washing-machine wastewater in Sfax, Tunisia. When this strain was grown in a medium containing 2% triacylglycerol, the hydrolysis products were found to be diacylglycerols, monoacylglycerols and free fatty acids. This strain was an aerobic, mesophilic, Gram-negative, motile, non-sporulating bacterium, capable of growing optimally at pH 7 and 27 degrees C. The predominant fatty acids were found to be C16:1omega7c (31%), C16:0 (28.1%), C18:1 omega7c (16.3%) and C17:0 (5.8%). Phylogenetic analysis of the 16S rRNA gene showed that this isolate is a new strain belonging to the genus Pseudomonas. Strain AHD-1 was found to be closely related to Pseudomonas azotoformans IAM 1603T, Pseudomonas gessardii CIP 105469T and Pseudomonas libanensis CIP 105460T with 99.7%, 99.56% and 99.54% of similarity, respectively.

摘要

从突尼斯斯法克斯经常受到洗衣机废水污染的土壤中分离到一株新型脂解细菌（菌株 AHD-1）。当该菌株在含有 2%三酰甘油的培养基中生长时，发现水解产物为二酰甘油、单酰甘油和游离脂肪酸。该菌株为需氧、中温、革兰氏阴性、运动、非孢子形成的细菌，能够在 pH7 和 27°C 的最佳条件下生长。优势脂肪酸为 C16:1omega7c（31%）、C16:0（28.1%）、C18:1 omega7c（16.3%）和 C17:0（5.8%）。16S rRNA 基因的系统发育分析表明，该分离株属于假单胞菌属的一个新菌株。菌株 AHD-1 与 Pseudomonas azotoformans IAM 1603T、Pseudomonas gessardii CIP 105469T 和 Pseudomonas libanensis CIP 105460T 具有 99.7%、99.56%和 99.54%的相似性。