序列决定核小体定位：最新进展与明显结局

Nucleosome positioning by sequence, state of the art and apparent finale.

机构信息

Genome Diversity Center, Institute of Evolution, University of Haifa, Mount Carmel, Haifa 31905, Israel.

出版信息

J Biomol Struct Dyn. 2010 Jun;27(6):741-6. doi: 10.1080/073911010010524944.

PMID:20232930

Abstract

All major suggestions about the nucleosome positioning sequence pattern(s) are overviewed. Two basic binary periodical patterns are well established: in purine/pyrimidine alphabet - YRRRRRYYYYYR and in strong/weak alphabet -SWWWWWSSSSSW. Their merger in four-letter alphabet sequence coincides with first ever complete matrix of nucleosome DNA bendability derived from very large database of nucleosome DNA sequences. Its simplified linear form is CGGAAATTTCCG. Several independent ways of derivation of the same pattern are described. It appears that the pattern represents an ultimate solution of long-standing problem of nucleosome positioning, and provides simple means for nucleosome mapping on sequences with single-base resolution.

摘要

概述了关于核小体定位序列模式的所有主要建议。已经确立了两种基本的二进制周期性模式：在嘌呤/嘧啶字母表中为 YRRRRRYYYYYR，在强/弱字母表中为 SWWWWWSSSSSW。它们在四字母字母序列中的合并与源自核小体 DNA 序列的非常大数据库的核小体 DNA 弯曲度的第一个完整矩阵相吻合。其简化的线性形式为 CGGAAATTTCCG。描述了几种推导相同模式的独立方法。该模式似乎代表了核小体定位这一长期问题的最终解决方案，并为在具有单碱基分辨率的序列上进行核小体作图提供了简单的方法。