Division of Parasitology, Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian, EH26 0PZ, Scotland, UK.
Mol Cell Probes. 2011 Oct-Dec;25(5-6):212-8. doi: 10.1016/j.mcp.2011.09.002. Epub 2011 Sep 22.
Early stages of sheep scab, the disease caused by the non-burrowing mite Psoroptes ovis, are often sub-clinical, or can be mis-diagnosed. A diagnostic test capable of detecting early disease and latent infestations is therefore highly desirable in disease control. This paper describes the design and validation of an ELISA, which incorporates a recombinant P. ovis antigen (Pso o 2), for the early detection of anti-P. ovis serum antibodies in sheep. This ELISA was evaluated using sera from sheep infested with P. ovis (n = 58) and sheep (n = 433) with no P. ovis infestation as well as sheep infected with other parasites including gastrointestinal nematodes (GIN), or chewing lice. A receiver operating characteristic (ROC) curve analysis was generated using the ELISA results for 491 sheep sera with the area under the curve (AUC) being 0.97. An optimal OD(450) cut-off of >0.06 absorbance units gave a test sensitivity of 0.93 and specificity of 0.90. The Pso o 2-based ELISA was able to detect specific antibodies to P. ovis during early experimental infestation prior to disease patency, indicating its utility for detecting sub-clinical infestation.
绵羊疥癣病（由非挖掘螨 Psoroptes ovis 引起）的早期阶段通常为亚临床症状，或者可能被误诊。因此，在疾病控制方面，非常需要一种能够检测早期疾病和潜伏感染的诊断检测方法。本文描述了一种 ELISA 的设计和验证，该 ELISA 采用了一种重组 P. ovis 抗原（Pso o 2），用于早期检测绵羊抗 P. ovis 血清抗体。该 ELISA 使用感染 P. ovis 的绵羊血清（n=58）和未感染 P. ovis 的绵羊血清（n=433）以及感染其他寄生虫（包括胃肠道线虫（GIN）或咀嚼虱）的绵羊血清进行了评估。使用 491 份绵羊血清的 ELISA 结果生成了受试者工作特征（ROC）曲线分析，曲线下面积（AUC）为 0.97。最佳 OD（450）截断值>0.06 吸光度单位时，检测的灵敏度为 0.93，特异性为 0.90。Pso o 2 基 ELISA 能够在疾病出现之前的早期实验感染期间检测到针对 P. ovis 的特异性抗体，表明其可用于检测亚临床感染。
