神圣罗勒的乙醇提取物通过抑制基质金属蛋白酶-9 和增强抗氧化酶来发挥抗转移活性。
Ethanol extract of Ocimum sanctum exerts anti-metastatic activity through inactivation of matrix metalloproteinase-9 and enhancement of anti-oxidant enzymes.
机构信息
Cancer Preventive Material Development Research Center, College of Oriental Medicine, Kyung Hee University, Seoul, South Korea.
出版信息
Food Chem Toxicol. 2010 Jun;48(6):1478-82. doi: 10.1016/j.fct.2010.03.014. Epub 2010 Mar 15.
Ocimum sanctum has been known to possess various beneficial properties including anti-oxidative, anti-inflammatory and anti-cancer activities. In the present study, we investigated that ethanol extracts of O. sanctum (EEOS) had anti-metastatic activity through activation of anti-oxidative enzymes. EEOS exerted cytotoxicity against Lewis lung carcinoma (LLC) cells. Also, EEOS significantly inhibited cell adhesion and invasion as well as activities of matrix metalloproteinase-9 (MMP-9), but not MMP-2, indicating the important role of MMP-9 in anti-metastatic regulation of EEOS. In addition, EEOS significantly reduced the tumor nodule formation and lung weight in LLC-injected mice. Inhibitory effect of EEOS on metastasis was further confirmed by using hematoxylin and eosin (H&E) staining. Notably, we also found that EEOS enhanced activities of anti-oxidative enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in a concentration-dependent manner. Taken together, our findings support that EEOS can be a potent anti-metastatic candidate through inactivation of MMP-9 and enhancement of anti-oxidant enzymes.
神圣罗勒已被证明具有多种有益特性，包括抗氧化、抗炎和抗癌活性。在本研究中，我们通过激活抗氧化酶来研究神圣罗勒的乙醇提取物（EEOS）具有抗转移活性。EEOS 对 Lewis 肺癌（LLC）细胞具有细胞毒性。此外，EEOS 还显著抑制细胞黏附和侵袭以及基质金属蛋白酶-9（MMP-9）的活性，但不抑制 MMP-2，表明 MMP-9 在 EEOS 的抗转移调节中起着重要作用。此外，EEOS 还显著减少了 LLC 注射小鼠的肿瘤结节形成和肺重。通过使用苏木精和伊红（H&E）染色进一步证实了 EEOS 对转移的抑制作用。值得注意的是，我们还发现 EEOS 能够浓度依赖性地增强超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）等抗氧化酶的活性。综上所述，我们的研究结果支持 EEOS 可以通过抑制 MMP-9 和增强抗氧化酶来成为一种有效的抗转移候选物。
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