用于区分马感染西尼罗河病毒和日本脑炎病毒的补体依赖细胞毒性试验。
Complement-dependent cytotoxicity assay for differentiating West Nile virus from Japanese encephalitis virus infections in horses.
作者信息
Kitai Yoko, Kondo Takashi, Konishi Eiji
机构信息
Department of International Health, Kobe University Graduate School of Health Sciences, Kobe, Japan.
出版信息
Clin Vaccine Immunol. 2010 May;17(5):875-8. doi: 10.1128/CVI.00217-09. Epub 2010 Mar 17.
Abstract
A complement-dependent cytotoxicity (CDC) assay was established to measure antibodies to the West Nile virus (WNV) nonstructural protein 1 (NS1) in horses. Sera collected from a WNV-infected horse mediated lysis of WNV NS1-expressing cells in a dose-dependent manner at higher percentages than sera from a Japanese encephalitis virus (JEV)-infected horse. The percentages of specific lysis for sera diluted 1:10 to 1:80 were <19.8% (assay cutoff) for almost all of the 100 JEV-infected or uninfected horses tested, in contrast to 55 to 76% in WNV-infected horses. Experimental infection revealed that horses became anti-WNV NS1 antibody positive 10 days after WNV infection. This study demonstrated the utility of this assay for differentiating WNV from JEV infections in horses.
摘要
建立了一种补体依赖性细胞毒性（CDC）试验，用于检测马体内针对西尼罗河病毒（WNV）非结构蛋白1（NS1）的抗体。从感染WNV的马身上采集的血清，以剂量依赖的方式介导了表达WNV NS1的细胞的裂解，其裂解百分比高于从感染日本脑炎病毒（JEV）的马身上采集的血清。在测试的100匹感染JEV或未感染JEV的马中，几乎所有血清稀释1:10至1:80后的特异性裂解百分比均<19.8%（试验临界值），相比之下，感染WNV的马的特异性裂解百分比为55%至76%。实验感染表明，马在感染WNV后10天抗WNV NS1抗体呈阳性。本研究证明了该试验在区分马感染WNV和JEV方面的实用性。
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