Department of Medicine (Gastroenterology), University of Alabama at Birmingham, Birmingham, Alabama, USA.
Mucosal Immunol. 2010 May;3(3):260-9. doi: 10.1038/mi.2010.10. Epub 2010 Mar 17.
Adaptive CD4 T-cell responses are important in the pathogenesis of chronic Helicobacter pylori gastritis. However, the gastric antigen-presenting cells that induce these responses have not yet been identified. Here we show that dendritic cells (DCs) are present in the gastric mucosa of healthy subjects and are more prevalent and more activated in the gastric mucosa of H. pylori-infected subjects. H. pylori induced gastric DCs isolated from noninfected subjects to express increased levels of CD11c, CD86 and CD83, and to secrete proinflammatory cytokines, particularly interleukin (IL)-6 and IL-8. Importantly, gastric DCs pulsed with live H. pylori, but not control DCs, mediated T-cell secretion of interferon-gamma. The ability of H. pylori to induce gastric DC maturation and stimulate gastric DC activation of Th1 cells implicates gastric DCs as initiators of the immune response to H. pylori.
适应性 CD4 T 细胞反应在慢性幽门螺杆菌胃炎的发病机制中很重要。然而，诱导这些反应的胃抗原呈递细胞尚未被鉴定。在这里，我们表明树突状细胞（DC）存在于健康受试者的胃黏膜中，并且在幽门螺杆菌感染受试者的胃黏膜中更为普遍和更活跃。幽门螺杆菌诱导从未感染受试者中分离出的胃 DC 表达更高水平的 CD11c、CD86 和 CD83，并分泌促炎细胞因子，特别是白细胞介素（IL）-6 和 IL-8。重要的是，用活幽门螺杆菌冲击的胃 DC 而非对照 DC 介导 T 细胞分泌干扰素-γ。幽门螺杆菌诱导胃 DC 成熟并刺激胃 DC 激活 Th1 细胞的能力表明胃 DC 是对幽门螺杆菌免疫反应的启动者。
