Tohda H, Oikawa A, Katsuki T, Hinuma Y, Seiji M
Cancer Res. 1978 Feb;38(2):253-6.
Nine lymphoblastoid cell lines were established after transformation by Epstein-Barr virus of peripheral lymphocytes from four xeroderma pigmentosum (XP) patients, the parents of one XP patient, and three normal donors. All these cell lines proliferate as suspension in Roswell Park Memorial Institute Medium 1640 supplemented with 20% fetal bovine serum, without detectable release of infectious Epstein-Barr virus. Some characteristics of these cell lines, such as growth rates, chromosome numbers, UV sensitivities, and activities of unscheduled DNA syntheses induced by UV, 4-nitroquinoline 1-oxide, and N-methyl-N'-nitro-N-nitrosoguanidine, were determined. Results confirm that the properties related to XP are not altered by transformation with Epstein-Barr virus and are the same in degrees of defect as are those of dermal fibroblasts from the respective individuals. These XP and normal lymphoblastoid cell lines should be especially useful for biochemical studies on the mechanism of DNA repair, because they are easy to grow in mass culture.
通过爱泼斯坦 - 巴尔病毒（Epstein-Barr virus）转化来自4名着色性干皮病（XP）患者、1名XP患者的父母以及3名正常供体的外周淋巴细胞，建立了9个淋巴母细胞系。所有这些细胞系均在添加20%胎牛血清的罗斯韦尔帕克纪念研究所培养基1640（Roswell Park Memorial Institute Medium 1640）中以悬浮状态增殖，未检测到传染性爱泼斯坦 - 巴尔病毒的释放。测定了这些细胞系的一些特性，如生长速率、染色体数目、紫外线敏感性以及由紫外线、4-硝基喹啉1-氧化物和N-甲基-N'-硝基-N-亚硝基胍诱导的非预定DNA合成活性。结果证实，与XP相关的特性不会因爱泼斯坦 - 巴尔病毒转化而改变，并且在缺陷程度上与各自个体的皮肤成纤维细胞相同。这些XP和正常淋巴母细胞系对于DNA修复机制的生化研究应该特别有用，因为它们易于在大规模培养中生长。
