Tohda H, Oikawa A, Kudo T, Tachibana T
Cancer Res. 1978 Oct;38(10):3560-2.
Ten lymphoblastoid cell lines were established by Epstein-Barr virus-induced transformation directly from 0.04 to 0.15 ml of peripheral whole blood of one patient with xeroderma pigmentosum and four normal healthy adults. All these lines expressed B-lymphocyte characteristics. The advantages of this method are: (a) only a few drops of blood are required for establishing a permanent line; (b) damage and loss of cells in separation procedures are minimal; and (c) the method is simple, reliable, and applicable, if desired, to any patient, even babies.
通过爱泼斯坦-巴尔病毒诱导转化，从一名着色性干皮病患者和四名正常健康成年人的外周全血中直接建立了10个淋巴母细胞系，所用血量仅为0.04至0.15毫升。所有这些细胞系均表现出B淋巴细胞特征。该方法的优点是：(a)建立永久性细胞系仅需几滴血液；(b)分离过程中细胞的损伤和损失最小；(c)该方法简单、可靠，如有需要，适用于任何患者，甚至婴儿。
