T抗原相关蛋白在SV40 DNA上的结合位点。

The binding site on SV40 DNA for a T antigen-related protein.

作者信息

Tjian R

出版信息

Cell. 1978 Jan;13(1):165-79. doi: 10.1016/0092-8674(78)90147-2.

PMID:202398

Abstract

A protein closely related to SV40 T antigen was purified in a biologically active form from cells infected with the defective adenovirus-SV40 hybrid, Ad2+D2. This 107,000 dalton hybrid protein binds and protects a specific portion of SV40 DNA from digestion by pancreatic DNAase I. Hybridization, endonuclease cleavage and pyrimidine tract analysis of the protected fragments reveal that the D2 hybrid protein binds in a sequential manner to tandem recognition sites which lie within a sequence of 120 nucleotides at position 67 near the origin of SV40 replication.

摘要

从感染缺陷腺病毒 - SV40 杂种病毒 Ad2 + D2 的细胞中，以生物活性形式纯化出一种与 SV40 T 抗原密切相关的蛋白质。这种 107,000 道尔顿的杂种蛋白质能结合并保护 SV40 DNA 的特定部分不被胰 DNA 酶 I 消化。对受保护片段的杂交、内切核酸酶切割和嘧啶序列分析表明，D2 杂种蛋白质以顺序方式结合到位于 SV40 复制起点附近 67 位的 120 个核苷酸序列内的串联识别位点上。