Tjian R, Fey G, Graessmann A
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1279-83. doi: 10.1073/pnas.75.3.1279.
Proteins related to simian virus 40 (SV40) T antigen uere isolated from cells infected with adenovirus 2/SV40 hybrids Ad2+D2 and Ad2+ND1 dp2 as well as from a line of human cells (SV80) transformed by SV40. The 96,000- and 107,000-dalton proteins of SV80 and Ad2+D2, after injection into the cytoplasm of cultured cells, rapidly accumulate in the nuclei, where they remain antigenically reactive for at least 20 hr and trigger DNA synthesis in quiescent cells. By contrast, the 23,000-dalton protein coded by Ad2+ND1 dp2 does not stimulate cellular DNA synthesis. However, all three purified proteins are able to provide helper function for the growth of adenovirus 2 in monkey cells. Thus, purified SV40 T antigen and proteins that share sequences with it retain the ability to carry out at least two functions associated with the product of the A gene of SV40.
从感染腺病毒2/SV40杂种病毒Ad2+D2和Ad2+ND1 dp2的细胞以及由SV40转化的人细胞系（SV80）中分离出与猿猴病毒40（SV40）T抗原相关的蛋白质。SV80和Ad2+D2的96,000道尔顿和107,000道尔顿蛋白质注射到培养细胞的细胞质中后，迅速在细胞核中积累，在那里它们至少20小时内保持抗原活性，并触发静止细胞中的DNA合成。相比之下，由Ad2+ND1 dp2编码的23,000道尔顿蛋白质不会刺激细胞DNA合成。然而，所有这三种纯化的蛋白质都能够为腺病毒2在猴细胞中的生长提供辅助功能。因此，纯化的SV40 T抗原及其共享序列的蛋白质保留了执行至少两种与SV40 A基因产物相关功能的能力。
