Price C P, Llyod B, Alberti G M
Clin Chem. 1977 Oct;23(10):1893-7.
We describe an automated kinetic assay for acetoacetate in blood. Acetoacetate is enzymatically reduced to D-beta-hydroxybutyrate and the reaction is monitored for 60 s with a reaction-rate analyzer. This technique allows low concentrations of acetoacetate to be measured with good precision and overcomes many of the problems associated with other automated techniques. Our studies on the stability of acetoacetate emphasize the need for care in handling specimens. The use of a reaction-rate analyzer, an item of equipment common to most laboratories, allows for rapid handling of samples in small or large batches, depending on the needs of the laboratory.
我们描述了一种用于血液中乙酰乙酸的自动动力学测定法。乙酰乙酸被酶促还原为D-β-羟基丁酸，并使用反应速率分析仪监测该反应60秒。该技术能够精确测量低浓度的乙酰乙酸，并克服了与其他自动化技术相关的许多问题。我们对乙酰乙酸稳定性的研究强调了在处理样本时需要小心谨慎。使用反应速率分析仪（大多数实验室都有的一种设备），可根据实验室的需求快速处理小批量或大批量样本。
