Työppönen J, Kauppinen K
Acta Vet Scand. 1980;21(1):55-61. doi: 10.1186/BF03546900.
An automated, spectro-photometric determination of blood acetoacetate and β-hydroxybutyrate was developed with a Gilford 3500 autoanalyzer. The stability of ketone bodies was studied in different conditions. An immediate precipitation with 0.6 M perchloric acid and cooling the sample effectively prevent the loss of acetoacetate from samples during transport to the laboratory (at 4°C a 6 % loss of acetoacetate was noted during 24 h). Freezing the sample makes it practically stable (less than 2 % loss of acetoacetate per week during a study lasting 2 months). At room temperature (20°C) the sample’s acetoacetate was instable and disappeared with a rate of 6 % per h. β-hydroxybutyrate was stable in precipitated samples. Because the precipitation also retains the sample’s glucose, 3 main parameters for the indication of ketosis could be analyzed automatically from the same sample with a total capacity of 40 samples in 2½ h.
利用吉尔福德3500自动分析仪开发了一种血液乙酰乙酸和β-羟基丁酸的自动分光光度测定法。研究了酮体在不同条件下的稳定性。用0.6 M高氯酸立即沉淀并冷却样品，可有效防止样品在运往实验室的过程中乙酰乙酸的损失（在4°C下，24小时内乙酰乙酸损失6%）。冷冻样品使其几乎稳定（在为期2个月的研究中，每周乙酰乙酸损失小于2%）。在室温（20°C）下，样品中的乙酰乙酸不稳定，以每小时6%的速率消失。β-羟基丁酸在沉淀样品中稳定。由于沉淀也保留了样品中的葡萄糖，因此可以在2个半小时内从同一样品中自动分析用于指示酮症的3个主要参数，总容量为40个样品。
